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尽管最近化疗和骨髓移植方法有所改进,急、慢性髓性白血病的预后仍不满意,肿瘤坏死因子(tumornecrosis factor alpha,TNF)是在移植了肿瘤的老鼠接种卡介苗(BCG)后注射内毒素,出现肿瘤的出血性坏死时发现的。一般认为 TNF 来源于致活的巨噬细胞。最近人类 TNF 的基因已被纯系化并已合成重组的蛋白。本文探讨了重组 TNF 对正常和恶性髓性细胞克隆生长的体外作用。9例髓性白血病的细胞系中有6例其细胞的克隆增殖被重组 TNF 所抑制,在软琼脂
Despite the recent advances in chemotherapy and bone marrow transplantation, the prognosis of acute and chronic myelogenous leukemia is still not satisfied. Tumor necrosis factor alpha (TNF) is injected into endotoxemia mice after BCG transplantation, Found when the tumor hemorrhagic necrosis. It is generally believed that TNF is derived from activated macrophages. Recently, the genes for human TNF have been purely made and recombinant proteins have been synthesized. This article explored the in vitro effects of recombinant TNF on clonal growth of normal and malignant myeloid cells. Six of nine myeloid leukemia cell lines were cloned and repressed by recombinant TNF and cultured on soft agar