论文部分内容阅读
研究表明,拟南芥中光敏色素介导的光信号与植物激素脱落酸(abscisic acid,ABA)途径相互作用.但水稻光敏色素与ABA途径之间是否相互影响仍不清楚.利用野生型和phyB突变体水稻作为研究材料,分析phyB介导的光信号对ABA生物代谢和ABA反应的影响.结果表明,ABA合成代谢相关基因OsNCED1,OsNCED2,OsNCED3和OsNCED4在phyB突变体中的表达水平明显高于野生型,而ABA降解代谢基因OsABAOX1则相反,这可能解释了phyB突变体积累较多内源ABA的原因.外源ABA处理明显抑制黑暗和光照下生长的水稻种子的萌发,但光照条件下ABA对phyB突变体种子萌发的抑制效果更明显.据此推测,phyB感受的光信号消弱了ABA对种子萌发的抑制效果.通过分析部分已报道的种子萌发相关基因在正常或ABA处理的野生型和phyB突变体中的表达水平,结果表明,phyB介导的光信号对水稻种子萌发的调控作用可能与这些基因无关.此外,在红光条件下,ABA处理能够抑制水稻幼苗地上部分的生长,野生型和phyB突变体对ABA处理的反应基本相同;但是ABA对phyB突变体主根生长的抑制效果显著高于野生型,这个结果表明phyB介导的光信号不影响ABA对水稻幼苗地上部分生长的抑制效果,但负调控ABA对主根生长的抑制效果.上述研究结果表明,phyB介导的光信号负调控水稻ABA的积累和ABA反应.本研究揭示了水稻光敏色素对ABA途径的影响,为深入研究光信号途径和ABA途径之间协同调控水稻发育的分子机制奠定了基础.
The results showed that phytochrome mediated Arabidopsis photosynthesis interacts with abscisic acid (ABA) pathway, but it is not clear whether phytochrome and ABA pathway interact with each other.With wild type and phyB Mutant rice was used as material to analyze the effect of phyB-mediated light signal on the ABA biosynthesis and ABA response.The results showed that the expression level of ABNC gene in the mutant of ABA was higher than that of OsNCED1, OsNCED2, OsNCED3 and OsNCED4 Wild-type, whereas the reverse transcription-abscisic acid gene, OsABAOX1, may account for the accumulation of more endogenous ABA in the phyB mutant.Exogenous ABA treatment significantly inhibited the germination of rice seeds grown under dark and light conditions, but ABA The inhibitory effect of phyB mutants on seed germination was more obvious.It was speculated that phyB sensitized light signal attenuated the inhibitory effect of ABA on seed germination.Based on the analysis of some reported seed germination-related genes in normal or ABA-treated wild type And phyB mutant.The results showed that phyB-mediated optical signal may play an important role in the regulation of rice seed germination.Therefore, ABA treatment inhibited the shoot growth of rice seedlings under the red light condition. The responses of wild-type and phyB mutants to ABA treatment were almost the same. However, the ABA treatment inhibited the growth of the main root of the phyB mutant more than the wild type. Indicating that phyB-mediated light signaling did not affect the inhibitory effect of ABA on the growth of rice shoots, but negatively regulated the inhibitory effect of ABA on the growth of main roots.The above results showed that phyB-mediated light signal negatively regulated the accumulation of ABA and ABA This study revealed the effects of rice phytochromes on ABA pathway, which lays the foundation for further study on the molecular mechanism of light signaling pathway and ABA pathway to coordinate the development of rice.