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目的:探讨瘦素在卵巢癌组织和癌旁组织、卵巢癌患者血清和尿液中的表达以及与卵巢癌发生、发展情况的关系。方法:用聚合酶链反应(PCR)和蛋白免疫印迹(Western Blot)法检测20例卵巢良性肿瘤组织、卵巢癌组织及相应癌旁组织中瘦素的表达,酶联免疫吸附实验(ELISA)测定25例卵巢癌患者术前和术后尿液中瘦素的表达,ELISA测定35例卵巢癌患者术前和术后血清中瘦素的表达。结果:卵巢癌组织中瘦素的表达水平明显高于癌旁组织和良性卵巢肿瘤组织中瘦素的表达,差异有统计学意义(P<0.01),卵巢癌患者血清和尿液中瘦素的表达水平术前高于术后,差异有统计学意义(P<0.01)。结论:瘦素在卵巢癌的发生、发展中可能起着一定的作用,但其具体的作用机制及是否可以作为卵巢癌诊断或预后的指标需进一步的研究。
Objective: To investigate the expression of leptin in ovarian cancer tissue and adjacent tissues, serum and urine of patients with ovarian cancer and the relationship with the occurrence and development of ovarian cancer. Methods: The expression of leptin was detected by polymerase chain reaction (PCR) and Western Blot in 20 cases of ovarian benign tumor tissues, ovarian cancer tissues and corresponding paracancerous tissues, and the enzyme linked immunosorbent assay (ELISA) The leptin expression in preoperative and postoperative urine of 25 patients with ovarian cancer and the serum leptin levels in 35 patients with ovarian cancer before and after surgery were determined by ELISA. Results: The expression of leptin in ovarian cancer tissues was significantly higher than that in adjacent non-cancerous tissues and benign ovarian tumor tissues (P <0.01). The levels of leptin in serum and urine of ovarian cancer patients The expression level before surgery was higher than that after surgery, the difference was statistically significant (P <0.01). Conclusion: Leptin may play a role in the occurrence and development of ovarian cancer, but its specific mechanism of action and whether it can be used as an indicator of ovarian cancer diagnosis or prognosis need further study.