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目的 :探讨干扰素α对白血病Raji细胞生长的抑制作用及其作用机制。方法 :以不同浓度的干扰素α作用于体外培养的Raji细胞 ,应用MTT法检测细胞生长抑制率 ,流式细胞仪检测细胞凋亡率 ,Hoechst 332 5 8荧光染色法观察细胞凋亡 ,TRAP PCR ELISA法检测细胞凋亡前后的端粒酶活性。结果 :5× 10 3 U·ml-1以上的干扰素α可显著降低Raji细胞端粒酶活性 ,抑制细胞的生长及诱导细胞发生凋亡 ,并呈现出明显的量 效与时 效关系。药物 (10×10 3 U·ml-1)作用 4 8～ 6 0h在Hoechst染色图片上可见核浓缩及核碎裂等典型的凋亡改变。结论 :干扰素α能抑制Raji细胞的生长并诱导细胞发生调亡 ,降低细胞端粒酶活性可能是其重要作用机制之一 ;这为干扰素应用于临床治疗淋巴瘤细胞白血病提供了有力的试验依据
Objective: To investigate the inhibitory effect of interferon α on the growth of leukemia Raji cells and its mechanism. Methods: Different concentrations of interferon α were used to effect Raji cells cultured in vitro. MTT assay was used to detect the cell growth inhibition rate. Flow cytometry was used to detect the apoptosis rate. Hoechst 332 58 staining was used to observe the apoptosis. TRAP PCR The telomerase activity before and after apoptosis was detected by ELISA. Results: Interferon α above 5 × 10 3 U · ml -1 could significantly decrease the telomerase activity, inhibit the cell growth and induce the apoptosis of Raji cells, and showed a significant dose-response and time-dependent effect. Drugs (10 × 10 3 U · ml-1) effect 4 8 ~ 60 h in the Hoechst staining showed nuclear condensation and nuclear fragmentation and other typical changes in apoptosis. CONCLUSION: Interferon α can inhibit the growth of Raji cells and induce the apoptosis of cells, and reduce the telomerase activity may be one of its important mechanisms. This provides a powerful test for the clinical application of interferon in the treatment of lymphoma cell leukemia in accordance with