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目的探究台湾海峡海洋沉积物中放线菌的多样性及发现合成药物先导化合物的新菌源。方法采用6种选择性培养基分离15份来自台湾海峡沉积物样品中含有的放线菌。挑选不同培养特征的放线菌进行初步分类鉴别、16S rRNA基因序列系统进化分析及基于PCR的烯二炔抗生素基因筛选。结果共分离到497株放线菌,挑选的95株放线菌分别属于放线菌7个科,11个属。16S rRNA基因序列分析结果提示分离到的小单孢菌科菌种存在数个潜在新种,95株菌中有27%的菌株含有烯二炔抗生素核弹头的生物合成基因片段。结论海洋环境蕴含丰富的放线菌资源,具有产生烯二炔类抗生素的潜能。
Objective To explore the diversity of actinomycetes in marine sediments in Taiwan Strait and to find out new source of synthetic compounds for lead compounds. Methods Six kinds of selective media were used to separate 15 strains of actinomycetes contained in the sediment samples from the Taiwan Strait. The actinomycetes with different culture characteristics were selected for preliminary classification and identification, phylogenetic analysis of 16S rRNA gene sequence and PCR-based screening of the gene of the enediyne antibiotics. Results A total of 497 strains of actinomycetes were isolated. The selected 95 strains of actinomycetes belong to 7 families and 11 genera, respectively. The results of 16S rRNA gene sequence analysis indicated that there were several potential new species of isolated Microtubugae strains and 27% of 95 strains contained biosynthetic gene fragments of the enendyne antibiotic nuclear warhead. Conclusion The marine environment is rich in actinomycetes resources, with the potential to produce enediyne antibiotics.