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目的探讨Dicer蛋白的表达与卵巢浆液性肿瘤发生、发展的关系及临床意义。方法选取1997年1月至2002年1月于中国医科大学附属盛京医院收住院行手术治疗并经术后病理证实的120例原发性卵巢浆液性癌病例,手术前均未进行放疗或化疗。正常卵巢组织9例、卵巢浆液性囊腺瘤13例和卵巢交界性浆液性囊腺瘤9例。以免疫组化方法检测Dicer蛋白在各组卵巢浆液性肿瘤组织中的表达情况。结果 (1)由正常卵巢组织向卵巢浆液性癌组织过渡过程中,Dicer蛋白阳性表达率呈下降趋势。其阳性表达在正常卵巢组织与浆液性囊腺癌组织(χ2=24.994,P<0.01)、浆液性囊腺瘤与浆液性囊腺癌组织(χ2=27.580,P<0.01)、交界性浆液性囊腺瘤与浆液性囊腺癌组织(χ2=13.180,P=0.01)中比较差异均有统计学意义。(2)Dicer蛋白表达在不同手术病理分期、组织学分级的卵巢浆液性癌组织中比较差异均有统计学意义(P均<0.05);在绝经前后和有无淋巴结转移中比较差异无统计学意义。结论 Dicer蛋白与卵巢浆液性肿瘤发生、发展有关,可能为卵巢恶性肿瘤的诊断和治疗提供新策略。
Objective To investigate the relationship between the expression of Dicer protein and the occurrence and development of ovarian serous tumors and its clinical significance. Methods From January 1997 to January 2002, Shengjing Hospital affiliated to China Medical University admitted surgical treatment of 120 cases of primary ovarian serous carcinoma confirmed by postoperative pathology cases, no radiotherapy or chemotherapy before surgery . 9 cases of normal ovarian tissue, 13 cases of ovarian serous cystadenoma and ovarian borderline serous cystadenoma in 9 cases. Immunohistochemistry was used to detect the expression of Dicer protein in ovarian serous tumors. Results (1) During the transition from normal ovarian tissue to ovarian serous carcinoma, the positive rate of Dicer protein showed a decreasing trend. (Χ2 = 24.994, P <0.01), serous cystadenoma and serous cystadenocarcinoma (χ2 = 27.580, P <0.01), the borderline serousity Cystadenoma and serous cystadenocarcinoma tissue (χ2 = 13.180, P = 0.01), the differences were statistically significant. (2) There was significant difference in the expression of Dicer between different surgical pathological stages and histological grade of ovarian serous carcinoma (all P <0.05). There was no significant difference between pre-menopausal and non-lymph node metastasis significance. Conclusion Dicer protein is related to the occurrence and development of ovarian serous tumors and may provide a new strategy for the diagnosis and treatment of ovarian malignant tumors.