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目的:探讨灵芝多糖成分(GLP)抑制肿瘤的作用机制。方法:在小鼠右腋皮下接种1×106TC-1细胞后7天后,用100mg/kg、200mg/kg和400mg/kg 3种剂量给小鼠口服灌胃给药20天,然后观察肿瘤的重量,并用ELISA检测小鼠血清中IL-2、IL-6和TNF-alpha,用流式细胞仪检测其外周血中CD4+和CD8+。结果:100mg/kg、200mg/kg和400mg/kg 3种剂量给小鼠口服灌胃给药20天,与对照组比较,抑瘤率分别可以达到53%、59%和58%,P<0.05;小鼠外周血血清中的IL-2从1.27ng/mL提高到了2.88ng/mL,P<0.05;TNF-α从1.05ng/mL提高到了1.82ng/mL,P<0.05;而IL-6则没有明显的变化。CD4+细胞水平升高(从54.80%提高到了58.27%),但差异无统计学意义(P>0.05);CD8+细胞明显增多(从24.15%提高到了45.36%),差异有统计学意义(P<0.05)。结论:GLP有明显抑瘤作用,但抑瘤作用与GLP剂量不存在依赖关系。GLP对肿瘤细胞生长的抑制是通过提高小鼠的细胞免疫能力来实现,而并非直接杀伤肿瘤细胞。
Objective: To investigate the mechanism of Ganoderma lucidum polysaccharide (GLP) inhibiting tumor. Methods: Mice were orally administered with three doses of 100mg / kg, 200mg / kg and 400mg / kg for 20 days 7 days after inoculation of 1x106TC-1 cells subcutaneously in the right axilla of mice, and then the weight of the tumor was observed The serum levels of IL-2, IL-6 and TNF-alpha were detected by ELISA. The levels of CD4 + and CD8 + in peripheral blood were detected by flow cytometry. Results: The mice were orally gavaged with three doses of 100mg / kg, 200mg / kg and 400mg / kg for 20 days. Compared with the control group, the tumor inhibition rates were 53%, 59% and 58% ; IL-2 in peripheral blood of mice increased from 1.27ng / mL to 2.88ng / mL, P <0.05; TNF-α increased from 1.05ng / mL to 1.82ng / mL, P <0.05; There is no obvious change. CD4 + cell levels (increased from 54.80% to 58.27%), but no statistical significance (P> 0.05) difference; CD8 + cells increased significantly (increase from 24.15% to 45.36%), the difference was significant (P <0.05 ). Conclusion: GLP has obvious anti-tumor effect, but there is no relationship between anti-tumor effect and GLP dose. The inhibition of the growth of tumor cells by GLP is achieved by increasing the cellular immunity of mice, rather than directly killing the tumor cells.