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报道了纳米级金膜微电极的制作方法.用XPS及SEM对电极表面进行了表征。考察了该电极的循环伏安及计时电流特性。在聚吡咯修饰微带金电极上成功地实现了葡萄糖氧化酶(GOD)和电子传递媒体Fe(CN)_6~(3-)的同时固定,并研究了GOD/Fe(CN)_6~(3-)/PPy微酶电极对葡萄糖的响应,稳态响应电流与葡萄糖浓度之间存在Michealis-Menten动力学特征。
The preparation method of nano-gold film microelectrode was reported.The surface of the electrode was characterized by XPS and SEM. The cyclic voltammetry and chronoamperometry of the electrode were investigated. The simultaneous immobilization of glucose oxidase (GOD) and electron transport mediator Fe (CN) 6 ~ (3-) on polypyrrole modified microstrip gold electrode was successfully studied. The effects of GOD / Fe -) / PPy micro-enzyme electrode response to glucose, Michelsis-Menten kinetic characteristics exist between steady-state response current and glucose concentration.