RP-HPLC法测定蒙药沙日崩-8汤中甘草酸的含量

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目的:测定沙日崩-8汤中甘草酸的含量。方法:甘草酸含量分析采用高效液相色谱法。色谱条件:乙腈-0.017mol/L磷酸溶液(35:65)为流动相。检测波长250nm,流速1.0ml/min。结果:样品中甘草酸达到基线分离;结果甘草酸在0.2372~2.3723μg范围内呈良好的线性关系,(r=0.99993)平均加样回收率为94.5%(RSD=0.67%)。结论:所建立的含量测定方法简便、准确、具有实用性。
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