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本研究采用主基因+多基因混合遗传模型分离分析法对大豆冠层的叶面积指数(leaf area index,LAI)、无截取散射(diffuse none-interceptance,DIFN)、平均叶倾角(mean leaf angle,MTA)3个参数进行遗传分析,并利用Win QTL Cartographer Version 2.5程序的复合区间作图法(CIM)及完备区间作图法QTL Ic IMapping软件进行QTL定位和上位性互作分析。染色体片段置换系(chromosome segment substitution lines,CSSL)群体冠层参数中,LAI最适模型为4MG-AI,DIFN最适模型为4MG-A,MTA两个最适模型为2MG-Duplicate和2MG-Inhibiting,重组自交系(recombinant inbred lines,RIL)群体冠层中LAI最适模型为4MG-EEEA,DIFN最适模型为4MG-A,MTA两个最适模型为2MG-Duplicate和2MG-Inhibiting。两套群体共检测到2个LAI相关的QTL位点,分别位于A1、I连锁群上。检测到22个与DIFN相关的QTL位点,分别位于A1、D1b、F、J、L、N、M、A2、K、B2、I、D1a连锁群上。检测到7个MTA相关的QTL位点,分别位于C2、E、I、A1、D1a连锁群上。CSSL群体冠层参数数据分析中发现,q LAI-A1-1和q DIF,N-A1-1同时在A1连锁群上的3 691~34 163 kb处检测到,q DIFN-A1-2和q MTA-A1-1同时在A1连锁群上的38 669~40 501 kb处检测到,两个位点可能为一因多效位点。仅在RIL群体冠层参数中发现6对QTL间存在着互作效应,在J、A1、B1、F、B2、E、D2连锁群上,在J连锁群上的q DIFN-J-1与A1连锁群上的q DIFN-A1-1和B1连锁群上的q DIFN-B1-1同时发生互作。
In this study, we analyzed the leaf area index (LAI), diffuse none-interceptance (DIFN) and mean leaf angle of soybean canopy using the combination of major gene plus polygene mixed genetic model MTA) were used to carry out genetic analysis. QTL mapping and epistasis interaction analysis were performed using the software of QTL Ic IMapping with the integrated interval mapping (CIM) software of the Win QTL Cartographer Version 2.5 and the complete interval mapping method. Among the canopy parameters of chromosome segment substitution lines (CSSL), the optimal model for LAI was 4MG-AI, the optimal model for DIFN was 4MG-A, the best model for MTA was 2MG-Duplicate and 2MG-Inhibiting The optimal model of LAI in the canopy of recombinant inbred lines (RIL) was 4MG-EEEA, the optimal model of DIFN was 4MG-A, and the two optimal models of MTA were 2MG-Duplicate and 2MG-Inhibiting. Two LAI-related QTLs were detected in the two groups, which were located on A1 and I linkage groups respectively. Twenty-two DIFN-related QTLs were detected on linkage groups A1, D1b, F, J, L, N, M, A2, K, B2, I and D1a, respectively. Seven MTA-related QTLs were detected on the linkage groups C2, E, I, A1 and D1a, respectively. In canopy profile data analysis of CSSL population, q LAI-A1-1 and q DIF and N-A1-1 were detected simultaneously at 3 691 ~ 34 163 kb on the A1 linkage group, q DIFN-A1-2 and q At the same time, MTA-A1-1 was detected at 38 669-40 501 kb on the A1 linkage group, and the two loci might be a multi-effect site. The interaction between 6 pairs of QTLs was found only in canopy parameters of RIL population. QDIFN-J-1 on J linkage group in linkage group J, A1, B1, F, B2, E, D2 and Q DIFN-A1-1 on the A1 linkage group and q DIFN-B1-1 on the B1 linkage group interact at the same time.