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玉米秸秆的氨水预处理在1.2L的高压反应器中进行。反应采用2个氨与生物质(玉米秸秆)的比例(质量比),分别为1:1和2:1;3个反应温度,分别为60℃,90℃和120℃;2个处理时间,分别为5分钟和30分钟。以水的预处理作为对照。用商用酶纤维素酶ACCELERASE 1500和木聚糖酶ACCELERASE XC使预处理的样品糖化成为可发酵糖。氨与生物质的质量比为1:1,90℃处理30分钟时,总的可发酵糖产量为87%。氨与生物质的质量比为2:1时,可发酵糖的产量是对照中可发酵糖分的4倍。氨与生物质的质量比2:1,90℃处理30分钟时,可发酵糖产量最多,达99%。本研究结果表明从玉米秸秆中提高可发酵糖的产量是非常有前景的。这一方法比氨法爆破和其他预处理方法如强酸强碱的预处理方法使用的氨剂量更小。然而,需要进一步优化实验以缩短预处理时间。
Ammonia water pretreatment of corn stover was carried out in a 1.2 L high pressure reactor. The reaction consisted of 1: 1 and 2: 1 ratio of two ammonia to biomass (corn stover), respectively. The three reaction temperatures were 60 ℃, 90 ℃ and 120 ℃, respectively. 5 minutes and 30 minutes respectively. Water pretreatment as a control. The pre-treated samples were saccharified to fermentable sugars with the commercial enzyme cellulase ACCELERASE 1500 and the xylanase ACCELERASE XC. The mass ratio of ammonia to biomass was 1: 1,900C for 30 minutes, and the total fermentable sugar yield was 87%. When the mass ratio of ammonia to biomass is 2: 1, the yield of fermentable sugar is four times that of the fermentable sugar in the control. Ammonia to biomass mass ratio 2: 1,90 ℃ for 30 minutes, the maximum yield of fermentable sugar, up to 99%. The results of this study show that increasing the yield of fermentable sugars from corn stover is very promising. This method uses less ammonia than the ammonia blasting and other pretreatment methods such as strong acid and alkali pretreatment methods. However, further optimization of the experiment is needed to shorten the pretreatment time.