【摘 要】
：
目的 观察自拟益气养血方对气血两虚型功能性便秘大鼠肠道菌群的影响.方法 将50只SD雄性大鼠随机分为空白组10只、造模组40只,以大黄灌胃联合尾静脉放血得到气血两虚型功能性便秘模型;将造模成功的40只大鼠,除将8只大鼠处死取材外,其余32只随机分为模型组、低剂量组、中剂量组和高剂量组各8只,干预4周.测量干预前后大鼠一般情况,观察记录大鼠体质量、粪便情况;通过OTU聚类分析方法、α多样性分析方法、β多样性分析方法分析肠道菌群多样性;通过SPSS分析比较不同干预方法下大鼠肠道菌群结构的差异.结果(1)治疗前
【机 构】
：
100078 北京中医药大学东方医院肛肠科
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目的 观察自拟益气养血方对气血两虚型功能性便秘大鼠肠道菌群的影响.方法 将50只SD雄性大鼠随机分为空白组10只、造模组40只,以大黄灌胃联合尾静脉放血得到气血两虚型功能性便秘模型;将造模成功的40只大鼠,除将8只大鼠处死取材外,其余32只随机分为模型组、低剂量组、中剂量组和高剂量组各8只,干预4周.测量干预前后大鼠一般情况,观察记录大鼠体质量、粪便情况;通过OTU聚类分析方法、α多样性分析方法、β多样性分析方法分析肠道菌群多样性;通过SPSS分析比较不同干预方法下大鼠肠道菌群结构的差异.结果(1)治疗前,与空白组相比,造模组体质量较低,粪便重量(干重和湿重)较低,干/湿比较高;治疗后,与模型组比较,采用自拟益气养血方治疗的各组大鼠精神、饮食等情况有所恢复,体质量较高,粪便重量(干重和湿重)较高,干/湿比较低,差异有统计学意义(P<0.05).(2)造模后,大鼠肠道菌群sobs指数、chao指数、ace指数、shannon指数降低,simpson指数升高;经过自拟益气养血方治疗后,ace指数、sobs指数、chao指数、shannon指数升高,simpson指数下降,差异具有统计学意义(P<0.05).(3)经过β多样性分析,造模后大鼠肠道菌群与造模前差异较大,经治疗后与空白组大鼠差异降低,高剂量组治疗后肠道菌群与空白组组间差异最小.(4)治疗后,门水平上,厚壁菌门、疣微菌门丰度降低,拟杆菌门、变形杆菌门、放射菌门等菌门丰度升高;属水平上,与模型组相比,低剂量组、中剂量组和高剂量组Blautia菌属、梭菌属、未分类的梭菌科、粪球菌属和瘤胃球菌属丰度较低,双歧杆菌属丰度较高,差异有统计学意义(P0.05).结论 益气养血方治疗功能性便秘不仅可改善大鼠的排便情况,还可以提高肠道菌群的多样性,提高有益菌的丰度,降低有害菌丰度.
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