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目的应用自动采样技术和传统采样方法对梓醇在大鼠体内的药动学进行研究,比较两种采样方法对梓醇药动学参数的影响。方法成年Wistar大鼠12只,随机分为2组,ig给予大鼠100mg/kg梓醇供试品后不同时间点分别采用自动采样技术与传统手动采样方法收集大鼠血样,经LC-MS/MS方法分析测得不同时间的血药浓度,应用DAS2.0计算程序、非隔室模型计算药动学参数并以单因素方差分析法对两种方法所得的药动学参数进行评价。结果大鼠ig给予梓醇供试品100mg/kg后,两组试验的血药浓度数据的变化趋势高度一致,两条平均药-时曲线趋势接近,达峰时间分别为1.5、1.0h,Cmax相差10%左右,计算所得各药动学参数单因素方差分析结果显示两组的各个参数均无显著差异。结论采用两种方法收集的血样分析计算所得的梓醇在大鼠体内药动学特征基本相同。
OBJECTIVE: To study the pharmacokinetics of catalpol in rats by using automatic sampling and traditional sampling methods. The effects of two sampling methods on pharmacokinetic parameters of catalpol were compared. Methods Twelve adult Wistar rats were randomly divided into two groups. Rats were given 100mg / kg catalpol at different time points. The blood samples were collected by automatic sampling and traditional manual sampling at different time points. The blood samples were collected by LC-MS / MS method was used to analyze plasma concentrations measured at different times. The DAS 2.0 calculation program was used to calculate the pharmacokinetic parameters in non-compartmental model. The pharmacokinetic parameters of two methods were evaluated by one-way ANOVA. Results After ig administration of catalpol to 100 mg / kg of test substance, the change trend of blood concentration data of the two groups was highly consistent. The trend of the two mean drug-time curves was close to 1.5, 1.0 h, Cmax A difference of about 10%, the calculated pharmacokinetic parameters of single-factor analysis of variance showed no significant differences in the two parameters of the two groups. Conclusion The pharmacokinetic characteristics of catalpol in rats obtained by the two methods are basically the same.