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目的筛选神经管缺陷(NTD)发生过程中与正常组织差异表达的细胞周期和细胞凋亡相关基因并作初步功能分析。方法用含有1 100余个已知基因的中密度芯片比较了胚胎9.5 d、10.5 d正常与同期NTD小鼠神经管组织的基因表达差异。结果通过比较正常E9.5 d与E10.5 d获得138个差异表达基因,E9.5 d-NTD获得20个差异,E10.5 d-NTD获得29个差异表达基因,根据基因功能分类包括细胞周期与凋亡相关基因,信号转导基因,转录与翻译调控,能量与代谢基因,热休克基因,基质与骨架蛋白等多种种类。而细胞周期和凋亡相关基因分别占差异基因的25.80%(32/124),45.00%(9/20)及28.57%(8/28)。结论实验证实多类基因参与了NTD的发生、发展过程,细胞周期和凋亡相关基因在神经管异常发育中具有重要作用,对该相关基因群的进一步研究有助于阐释NTD的发病机制。
Objective To screen the cell cycle and apoptosis-related genes differentially expressed in normal tissues during the development of neural tube defects (NTDs) and to make preliminary functional analysis. Methods The gene expression of neural tube in normal and NTD mice at embryonic 9.5 d and 10.5 d was compared using a medium density chip containing more than 1,100 known genes. Results 138 differentially expressed genes were obtained by comparing normal E9.5 d and E10.5 d with 20 differences in E9.5 d-NTD and 29 differentially expressed genes in E10.5 d-NTD. According to the gene function classification, including cells Cycle and apoptosis related genes, signal transduction genes, transcription and translation regulation, energy and metabolism genes, heat shock genes, matrix and scaffold proteins and other species. The cell cycle and apoptosis related genes accounted for 25.80% (32/124), 45.00% (9/20) and 28.57% (8/28) of the differentially expressed genes, respectively. CONCLUSION: Many kinds of genes have been involved in the genesis and development of NTD. Cell cycle and apoptosis-related genes play an important role in the abnormal development of neural tube. Further study on the related gene cluster may help explain the pathogenesis of NTD.