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目的:探讨细胞色素P450 1A1(CYP1A1)、依赖还原型辅酶Ⅰ/Ⅱ醌氧化还原酶1(NQO1)和环氧化物水解酶(EPHX1)基因多态性与浙江地区膀胱癌易感性的关系。方法:采用病例对照研究方法,应用相对的2对引物-聚合酶链反应技术(PCR-CTPP),对99例膀胱癌患者和100例非肿瘤患者的CYP1A1A4889G、NQO1C609T和EPHX1A416G基因型进行检测,并分析其与膀胱癌易感性的关系。结果:NQO1C609T基因型分布频率在膀胱癌组和对照组之间的差异有统计学意义,P<0.05;携带609TT基因型的个体罹患膀胱癌的风险是携带609CC基因型个体的2.448倍(95%CI为1.125～5.326)。CYP1A1A4889G和EPHX1A416G基因型分布频率在两组间的差异均无统计学意义,P>0.05。携带NQO1 609TT与EPHX1 416AA两高危基因型的个体罹患膀胱癌的风险是同时携带两低危基因型个体的4.181倍(95%CI为1.465～11.934)。相类似的,携带CYP1A1 4889AG/GG、NQO1 609TT与EPHX1 416AA三高危基因型的个体罹患膀胱癌的风险则为4.765倍(95%CI为0.895～25.364)。结论:NQO1C609T基因多态性可能与膀胱癌的易感性相关,携带NQO1 609TT基因型的人群易患膀胱癌。而且,基因与基因(NQO1 609TT与EPHX1 416AA,CYP1A1 4889AG/GG、NQO1 609TT与EPHX1 416AA)的交互作用增加了这种风险。
Objective: To investigate the relationship between CYP1A1, NQO1 and EPHX1 gene polymorphisms and susceptibility to bladder cancer in Zhejiang Province. Methods: The CYP1A1A4889G, NQO1C609T and EPHX1A416G genotypes in 99 bladder cancer patients and 100 non-tumor patients were detected by case-control study using two pairs of primer-polymerase chain reaction (PCR-CTPP) Analysis of its relationship with bladder cancer susceptibility. Results: The frequency of NQO1C609T genotype was significantly different between bladder cancer group and control group (P <0.05). The risk of bladder cancer in individuals carrying 609TT genotype was 2.448 times (95%) than those carrying 609CC genotype CI 1.125 ~ 5.326). There was no significant difference in the distribution frequency of CYP1A1A4889G and EPHX1A416G between the two groups (P> 0.05). Individuals with two high-risk genotypes, NQO1 609TT and EPHX1 416AA, had a 4.181-fold (95% CI, 1.465 to 11.934) risk of developing bladder cancer compared with individuals with two low-risk genotypes. Similarly, individuals with CYP1A1 4889AG / GG, NQO1 609TT and EPHX1 416AA three-risk genotypes had a 4.765-fold risk of developing bladder cancer (95% CI, 0.895-25.364). Conclusion: The polymorphism of NQO1C609T may be associated with the susceptibility of bladder cancer. The population with NQO1 609TT genotype is predisposed to bladder cancer. Moreover, the interaction of genes with genes (NQO1 609TT and EPHX1 416AA, CYP1A1 4889AG / GG, NQO1 609TT and EPHX1 416AA) increases this risk.