论文部分内容阅读
苍耳子仁粉碎后用０．１５ｍｏｌ·Ｌ－１ＮａＣｌ、０．００１ｍｏｌ·Ｌ－１ｐＨ７．０磷酸缓冲液抽提，硫酸铵沉淀及乳糖－淀粉凝胶柱亲和层析，分离纯化得到苍耳子凝集素。经聚丙烯酰胺凝胶电泳，考马斯亮蓝Ｒ２５０染色，显示一条蛋白带，其溶液用苯酚－硫酸显色证明是糖蛋白。苍耳子凝集素使新鲜兔红细胞产生血凝作用所需最低浓度为６．８μｇ·ｍｌ－１。苍耳子凝集素的专一结合的糖为２－乙酰氨基－２－脱氧－Ｄ－半乳糖、棉子糖、乳糖和半乳糖。它对人Ａ、Ｂ、Ｏ型血和人精子均有凝集作用。苍耳子凝集素具有较强的耐热性，反复冻融对其活性影响甚小。
The cocklebur seeds were crushed and extracted with 0.15 mol·L-1 NaCl, 0.001 mol·L-1 pH 7.0 phosphate buffer, ammonium sulfate precipitation and lactose-starch gel affinity chromatography, and the Xanthium spp. was isolated and purified. Sublectin. On polyacrylamide gel electrophoresis, Coomassie Brilliant Blue R250 was stained to show a protein band, and the solution was visualized with phenol-sulfuric acid to show that it was a glycoprotein. The lowest concentration of Xanthium lectin required for hemagglutination of fresh rabbit erythrocytes was 6.8 μg·ml-1. The specifically bound sugars of Xanthium lectin are 2-acetylamino-2-deoxy-D-galactose, raffinose, lactose and galactose. It has agglutination of human A, B, O blood and human sperm. Xanthium lectin has strong heat resistance, and repeated freezing and thawing has little effect on its activity.