论文部分内容阅读
目的为研究真核细胞过氧化适应反应及其可能机理。方法在中国仓鼠肺成纤维细胞（ＣＨＬ）上，通过低剂量Ｈ２Ｏ２预处理，观察细胞和ＤＮＡ对高剂量Ｈ２Ｏ２再处理的适应反应，同时测定ＣＡＴ酶和ＰＡＲＰ的变化及其在适应ＤＮＡ断裂中的作用。结果在细胞活力和ＤＮＡ断裂水平上均观察到非致死性适应反应，然而这两种水平的适应模式（条件）有所不同，提示两者可能存在不同的诱导适应的途径。低剂量Ｈ２Ｏ２可诱导ＣＡＴ酶活性增高，并与抑制ＤＮＡ断裂的适应效应具有相关性（ｒ＝０．９５，Ｐ＜０．０５），同时ＰＡＲＰ阻断剂３氨基苯胺（３ＡＢ）可明显抑制ＤＮＡ断裂水平的适应反应。结论ＣＨＬ细胞系存在细胞活力变化和ＤＮＡ断裂的过氧化非致死性适应。ＣＡＴ和ＰＡＲＰ参与过氧化ＤＮＡ断裂的适应诱导
Objective To study the eukaryotic cells peroxidation response and its possible mechanism. Methods Hamster lung fibroblasts (CHL) were pretreated with low dose of H2O2 to observe the adaptive response of cells and DNA to high dose H2O2 reprocessing. Meanwhile, the changes of CAT and PARP and their roles in DNA fragmentation effect. Results Non-lethal adaptive responses were observed both at cell viability and DNA fragmentation. However, the adaptation patterns (conditions) at these two levels were different, suggesting that there may be different ways of inducing adaptation between the two. Low-dose H2O2 could induce the increase of CAT activity, which was correlated with the adaptive effect of inhibiting DNA fragmentation (r = 0.95, P <0.05). PARP blocker 3-aminoaniline (3AB) Appropriate inhibition of DNA fragmentation response to the level of adaptation. Conclusion CHL cell lines exist cell viability changes and DNA fragmentation peroxidation non-lethal adaptation. CAT and PARP are involved in adaptive induction of DNA fragmentation