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目的调查唐山地区A组轮状病毒感染现况。方法 RT-PCR鉴定唐山地区A组轮状病毒流行株型别,提取病毒总RNA,扩增目的基因片段VP7,分离纯化和回收,连接pMD18-T载体,转化质粒进行抽提与鉴定,同时进行基因测序。结果与标准病毒株进行基因差异分析。结果成功连接VP7基因与pMD18-T载体。基因测序分析结果 :唐山地区A组轮状病毒VP7基因与标准病毒株VP7基因序列符合率(同源性)为98.5%,其关键基因没有发生变异。结论唐山地区A组轮状病毒VP7基因的关键基因没有发生变异。
Objective To investigate the status of group A rotavirus infection in Tangshan area. Methods The rotavirus strains of group A in Tangshan area were identified by RT-PCR. The total RNA was extracted and the target gene fragment VP7 was amplified. The recombinant plasmid was isolated and purified. The vector pMD18-T was ligated and transformed into plasmids for extraction and identification. Gene Sequencing. Results Gene mutation analysis was performed with standard strains. Results The VP7 gene and pMD18-T vector were successfully ligated. Results of sequencing analysis showed that the coincidence rate (homology) between the VP7 gene of group A rotavirus and that of the standard strain VP7 was 98.5% in Tangshan area, and the key genes did not change. Conclusion There is no variation in the key genes of VP7 gene of group A rotavirus in Tangshan area.