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目的 研究神经元型一氧化氮合酶（ｎＮＯＳ）抑制剂７－硝基吲唑（７－ＮＩ）对大鼠异氟醚ＭＡＣ的影响。方法 ３０只雄性ＳＤ大鼠，随机分为６ 组（每组５ 只）。大鼠置于密闭麻醉系统中，按标准夹尾法测定异氟醚ＭＡＣ。每只鼠测定基础ＭＡＣ后，随机经腹腔注射３ｍ ｌ花生油，或２０、６０、８０、１２０或２５０ｍ ｇ·ｋｇ－ １ ７－ＮＩ（溶于３ｍ ｌ花生油），３０ 分钟后测定ＭＡＣ值。选１２０ｍ ｇ·ｋｇ－ １ ７－ＮＩ组大鼠再腹腔注射５００ｍ ｇ·ｋｇ－ １Ｌ－精氨酸，１５分钟后测定ＭＡＣ值。结果 ２０～２５０ｍ ｇ·ｋｇ－ １ ７－ＮＩ显著降低异氟醚ＭＡＣ，分别较基础ＭＡＣ下降（１５．６±２．３）％ 、（２１．９±２．１）％ 、（２９．９±２．５）％ 、（３０．４±１．７）％ 、（２９．８±３．３）％ ，当７－ＮＩ剂量超过８０ｍ ｇ·ｋｇ－ １时出现封顶效应。５００ｍ ｇ·ｋｇ－ １ Ｌ－精氨酸可完全逆转１２０ｍ ｇ·ｋｇ－ １ ７－ＮＩ降低ＭＡＣ效应。结论 抑制中枢ｎＮＯＳ活性，可增强异氟醚麻醉效能，提示中枢ＮＯ信息途径可能在全麻药作用分子机制中起作用。
Objective To investigate the effect of 7-nitroindazole (7-NI), a neuronal nitric oxide synthase (nNOS) inhibitor, on isoflurane MAC in rats. Methods Thirty male SD rats were randomly divided into 6 groups (5 in each group). Rats were placed in a closed anesthesia system, according to standard clamp tail method for the determination of isoflurane MAC. After each rat was assayed for basal MAC, MACs were randomly injected either 3 ml peanut oil or 20, 60, 80, 120 or 250 mg kg-1 7-NI (dissolved in 3 ml peanut oil) per ip. The rats in the 120 m g · kg-1 7-NI group were injected intraperitoneally with 500 mg · kg -1 L-arginine, and MAC values were measured after 15 minutes. Results The MAC of isoflurane decreased significantly from 20 to 250 m g · kg-1 7-NI (15.6 ± 2.3%, 21.9 ± 2.1%, 29.9 ± 2.5%, (30.4 ± 1.7)% and (29.8 ± 3.3)%, respectively. The capping effect occurred when the dose of 7-NI exceeded 80 m g · kg-1. 500m g · kg -1 L-arginine can completely reverse 120m g · kg-1 7-NI reduce MAC effect. Conclusion Inhibition of central nNOS activity enhances the anesthetic efficacy of isoflurane, suggesting that the central NO signaling pathway may play a role in the molecular mechanism of general anesthetic action.