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假如血糖含量超过340毫克％,则不能按上述方法进行检查,因为不能剩余过多的铁氰化钾。加两次铁氰化钾亦不能获得正确的结果,因为铁氰化钾还元量呈抛物線状增加,因此血糖愈多则愈不易计算。假设铁氰化钾的还元是在良好的反应环境条件下,还可将铁氰化钾成直線增加。为此将试剂在(?)酸盐的缓冲溶液中制备。试剂 (1)及(2)如前法制备。(3)[甲]燐酸盐缓冲溶液:将70克燐酸二钾与21.25克燐酸三钾溶解於1升量瓶的水中,加水至刻度;(3)[乙]将3.3克铁氰化钾溶解於1升的量瓶中,加水至刻度。使用前将(3)[甲]与(3)[乙]等量混合。试剂(4)、(6)、(7)
If the blood glucose level exceeds 340 mg%, the test cannot be performed as described above because excessive potassium ferricyanide cannot be left. The addition of two potassium ferricyanides also failed to obtain the correct result, because the amount of potassium ferricyanide increased parabolically, so the more blood sugar the more difficult to calculate. Assuming that the potassium ferricyanide is in a good reaction environment, potassium ferricyanide can also increase linearly. For this purpose, the reagents are prepared in a buffer solution of the (Na) acid salt. Reagents (1) and (2) were prepared as before. (3) [A] citrate buffer solution: Dissolve 70 grams of dipotassium citrate and 21.25 grams of tripotassium citrate in a 1 liter flask of water and add water to the mark; (3) [B] Dispose 3.3 grams of potassium ferricyanide Dissolve in a 1 liter volumetric flask and add water to the mark. Mix (3) [A] with (3) [B] equal amounts before use. Reagents (4), (6), (7)