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目的观察大黄酸对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠肾脏中神经肽Y(neuropeptideY,NPY)及其Y1(NPY1R)、Y2(NPY2R)受体表达的影响。方法 STZ诱导糖尿病大鼠模型,将糖尿病大鼠随机分为糖尿病对照组和大黄酸治疗组,并以正常对照组作为对照,每组动物12只。大黄酸治疗组给予100mg.kg-1.d-1大黄酸灌胃,其他两组给予相同体积的溶剂灌胃,共10周。运用放射免疫法测定NPY浓度,荧光定量逆转录聚合酶链反应测定NPY及其Y1、Y2受体基因表达水平,Western印迹法检测Y1、Y2受体蛋白表达水平。结果大黄酸可降低糖尿病大鼠血糖、甘油三酯、总胆固醇、24h蛋白尿水平(P<0.01),缓解糖尿病肾病的病理变化。与正常对照组相比,糖尿病大鼠下丘脑和肾脏中NPY浓度明显升高(P<0.01),肾脏中Y1和Y2受体的mRNA和蛋白表达增加(P<0.05);大黄酸干预后NPY及其Y1、Y2受体表达量显著低于糖尿病组(P<0.05)。结论大黄酸对STZ诱导的糖尿病大鼠糖脂代谢紊乱、肾脏病变有一定程度的保护作用,可能与其调节神经肽Y及其Y1、Y2受体的表达有一定关系。
Objective To investigate the effect of rhein on the expression of neuropeptide Y (NPY) and its receptors Y1 (NPY1R) and Y2 (NPY2R) in the kidney of streptozotocin-induced diabetic rats. Methods STZ induced diabetic rat model, diabetic rats were randomly divided into diabetic control group and rhein treatment group, and the normal control group as a control, 12 animals in each group. Rhein treatment group given 100mg.kg-1.d-1 rhein administration, the other two groups given the same volume of solvent gavage for 10 weeks. The concentration of NPY was determined by radioimmunoassay. The expression of NPY and Y1, Y2 receptor mRNA was detected by fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR). The expression of Y1 and Y2 receptor protein was detected by Western blotting. Results Rhein can reduce blood glucose, triglyceride, total cholesterol and proteinuria in diabetic rats (P <0.01), and alleviate the pathological changes of diabetic nephropathy. Compared with normal control group, the concentration of NPY in hypothalamus and kidney of diabetic rats was significantly increased (P <0.01), and the mRNA and protein expression of Y1 and Y2 receptors in kidney increased (P <0.05). After rhein intervention, NPY The expression of Y1 and Y2 receptors was significantly lower in diabetic group than in diabetic group (P <0.05). Conclusion Rhein has a protective effect on glucose and lipid metabolism and kidney disease induced by STZ in diabetic rats, which may be related to the regulation of neuropeptide Y and its receptors of Y1 and Y2.