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高压氧的作用曾经在活体内与活体外研究,并得到一个概念,即高压氧可影响增生和新生组织的血管形成,故可利用高压氧的特性作用于治疗,特别是阻止一些病理情况下血管的生长,作者试图证明高压氧对活动性生长的血管的作用而进行了动物实验。因为正常角膜无血管,无论在活体内或活体外,新增生的血管的反应易于观察,故以角膜为研究材料。选16只已长成的豚鼠,右眼前房内注入四氧嘧啶。注射后5～7天角膜新生血管明显,将8只试验动物放于持续供应1.5～2个大气压的100%氧气的加压室内。对照组8只豚鼠放于原饲养室。在高压氧室内17～37小时后,豚鼠发生严重的呼吸困难。此时给予缓慢减压,后移至大气压下,用手持裂隙灯和双眼显微镜检查眼部情况。所有氧气治疗的动物自加压室移出后,数小时内自然死亡,死后取出肺脏检查。将墨水自左心室注入,直至角膜血管充满墨水为止,摘出眼球固定。当一只氧气治疗组的豚鼠死亡时,同时将对照组的一只豚鼠经腹膜注入戊巴比妥钠杀死,
The role of hyperbaric oxygen has been studied in vitro and in vivo with the notion that hyperbaric oxygen can affect hyperplasia and neovascularization of blood vessels and therefore can take advantage of the properties of hyperbaric oxygen to treat, in particular, the prevention of vascular pathology The authors attempted to demonstrate the effect of hyperbaric oxygen on actively growing blood vessels in animal experiments. Because normal corneal avascular, both in vivo or in vitro, the new blood vessels of the reaction is easy to observe, so the cornea as the research material. Select the guinea pigs have grown into 16, the right anterior chamber injection of alloxan. Corneal neovascularization was evident 5 to 7 days after injection and 8 experimental animals were placed in a pressurized chamber with 100% oxygen being continuously supplied at 1.5 to 2 atm. Control group of 8 guinea pigs placed in the original breeding room. After 17 to 37 hours in the hyperbaric chamber, guinea pigs developed severe dyspnea. At this time to give a slow decompression, after moving to atmospheric pressure, with hand-held slit lamp and binocular microscope examination of the eye situation. All oxygen-treated animals died spontaneously within a few hours after they were removed from the compression chamber and were taken out of the lungs after death. The ink is injected into the left ventricle until the corneal blood vessels are filled with ink, and the eyeballs are removed. When a guinea pig in the oxygen treatment group died, at the same time a guinea pig in the control group was killed by peritoneal injection of sodium pentobarbital,