论文部分内容阅读
目的:探讨脂多糖(lipopolysaccharide,LPS)对人牙髓干细胞(human Dental pulp Stem cells,hDPSCs)增殖及骨向分化的作用关系.方法:将第3代hDPSCs体外常规培养后,采用MTT法分别检测不同浓度LPS(0、1、10、100、1000 ng/mL)作用hDPSCs 72 h后细胞增殖情况,筛选出促进hDPSCs增殖的LPS最佳浓度.通过Western Blot法检测该浓度作用下hDPSCs中各矿化相关蛋白:碱性磷酸酶(ALP)、骨钙素(OCN)、骨唾液酸蛋白(BSP)及牙本质涎磷蛋白(DSPP)的表达水平.结果:与对照组比较,1 ng/mL及10 ng/mL LPS干预组能明显促进hDPSCs的增殖(P<0.05),其中以10 ng/mL LPS作用更显著.Western Blot检测结果表明,10 ng/mL LPS可明显上调hDPSCs中各矿化相关蛋白的表达水平.结论:低浓度LPS可促进hDPSCs的增殖和骨向分化.“,”Objective:To explore the effect of lipopolysaccharide(LPS)in the regulation of proliferation and osteo-genic differentiation of human dental pulp stem cells.Methods:The third generation of human dental pulp stem cells were cultured in vitro and treated with LPS for 72 hours at different concentrations of LPS(0,1,10,100 and 1000 ng/mL)respec-tively.MTT assay was employed to evaluate the optimum concentration of LPS for promotion cell proliferati on.The protein ex-pression level of ALP,OCN,BSP and DSPP in hDPSCs treated with low concentration of LPS were examined respectively by Western Blot.Results:The proliferation of hDPSCs were obviously induced by low concentration of LPS(1 ng/mL and 10 ng/mL),and 10 ng/mL LPS was better to promote cell proliferation.Western Blot results showed that 10 ng/mL LPS up-regula-ted expression of osteogenic differentiation related proteins.Conclusion:LPS can enhance proliferation and osteogenic differ-entiation of hDPSCs at low concentration.