论文部分内容阅读
WRKY是植物中最大的转录因子家族之一.本文对水稻WRKY42基因的转录分析发现,该基因在水稻苗期和花药中转录,其蛋白质可在各时期的叶片中检测到.在Xa21介导的抗白叶枯病过程中,接菌后期可检测到明显的诱导表达条带,比较其在抗、感和对照反应中的表达丰度发现,在抗、感反应中的表达相似但均明显大于对照反应,推测WRKY42蛋白质在水稻-白叶枯病菌互作反应中发挥作用.我们克隆并在细菌中表达了WRKY42蛋白质,采用微量热泳动(microscale thermophoresis)技术,调查了WRKY42与病程相关基因PR1a和PR1b启动子区顺式元件W-box的互作,发现它们之间可发生特异结合,其解离常数分别为73.3μmol/L和58.3μmol/L.上述数据提供了WRKY42调控下游基因的直接证据,支持WRKY42在水稻抗病过程中发挥作用.文章提出了WRKY转录因子在水稻与白叶枯病菌互作过程中的作用模式.
WRKY is one of the largest family of transcription factors in plants.In this paper, transcriptional analysis of rice WRKY42 gene revealed that the gene was transcribed in rice seedling and anther, and its protein was detected in leaves of different stages.While Xa21-mediated In the process of bacterial blight resistance, the obvious induced expression bands could be detected in the post-inoculation stage. Compared with the expression abundance in anti-sense and control reactions, the expression levels in anti-bacterial and sensory responses were similar but significantly greater than The control reaction suggested that WRKY42 protein plays a role in the interaction between rice and bacterial blight.We cloned WRKY42 protein and expressed WRKY42 protein in bacteria and investigated the relationship between WRKY42 and PR1a And PR1b promoter cis-element W-box interaction and found that they can occur between the specific binding dissociation constants were 73.3μmol / L and 58.3μmol / L. These data provide WRKY42 regulation of downstream genes directly The evidence supports that WRKY42 plays a role in disease resistance in rice.This paper presents the role of WRKY transcription factor in the interaction between rice and bacterial leaf blight.