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目的:检测不同年龄组正常人避光部位及曝光部位表皮组织中亲环素A(CyPA)和CD147的表达,初步探讨CyPA和CD147在皮肤衰老过程中的意义。方法:收集少年组(<15岁)、中年组(30-40岁)和老年组(>65岁)避光部位和曝光部位正常皮肤各20例,运用免疫组织化学及原位杂交2种方法检测表皮中CyPA和CD147蛋白和mRNA的表达水平,并分析各年龄组CyPA和CD147的表达水平的差异及相关性。结果:免疫组织化学结果显示CyPA和CD147 2种蛋白在曝光和避光部位都为老年组最高,中年组次之,少年组最低,各组间差异具有统计学意义(均P<0.05),且各组中CyPA和CD147表达呈正相关(避光部位r=0.899,曝光部位r=0.945)。组织原位杂交结果显示CyPA和CD147 mRNA在曝光和避光部位都为老年组最高,中年组次之,少年组最低,各组间差异具有统计学意义(均P<0.05),且各组中CyPA和CD147表达呈正相关(避光部位r=0.792,曝光部位r=0.782)。结论:CyPA和CD147相互作用可能在皮肤衰老过程中起重要作用。
Objective: To detect the expression of cyclophilin A (CyPA) and CD147 in the dark spots and the epidermis of normal subjects in different age groups, and to explore the significance of CyPA and CD147 in the process of skin aging. Methods: Twenty cases of normal skin were collected from juvenile group (<15 years old), middle aged group (30-40 years old) and senile group (> 65 years old), respectively. Immunohistochemistry and in situ hybridization Methods The expressions of CyPA and CD147 protein and mRNA in the epidermis were detected and the differences and correlations between the expressions of CyPA and CD147 in different age groups were analyzed. Results: The results of immunohistochemistry showed that CyPA and CD147 2 proteins were the highest in the aged group and the middle aged group, the lowest in the juvenile group, and the difference between the groups was statistically significant (all P <0.05) The positive expression of CyPA and CD147 in each group (r = 0.899 for the dark area and r = 0.945 for the exposed area). Tissue in situ hybridization results showed that CyPA and CD147 mRNA were the highest in the aged group and the middle aged group, the lowest in the juvenile group, the difference between the groups was statistically significant (all P <0.05), and each group There was a positive correlation between CyPA and CD147 expression (r = 0.792 in the dark and r = 0.782 in the exposed area). Conclusion: The interaction between CyPA and CD147 may play an important role in the process of skin aging.