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目的:建立测定白木香叶和远志地上各部分(包括远志整个地上部分、远志叶和远志茎)芒果苷含量的高效液相色谱法,为寻找富含芒果苷的植物资源奠定基础。方法:采用Kromasil 100C18(4.6 mm“250 mm,5μm)色谱柱,以乙腈-0.5%磷酸溶液(15∶85)为流动相,等度洗脱10 min,流速为1 mL.min-1,检测波长为258 nm,柱温为30℃。结果:在本色谱条件下,芒果苷和样品中其他成分分离良好,并且浓度在0.116～580μg.mL-1范围内线性良好。加样回收率(n=6)分别为97.5%(白木香叶),97.3%(远志地上部分),97.1%(远志叶),98.5%(远志茎);RSD分别为1.6%,2.1%,1.8%,0.21%。结论:本方法操作简单、快捷、准确,重复性好,可用于测定白木香叶、远志地上部分等不同来源植物中芒果苷的含量。
OBJECTIVE: To establish a HPLC method for the determination of mangiferin in ground parts of Polygala tenuifolia and Polygala tenuifolia (including the above-ground part, Polygala tenuifolia and Polygala tenuifolia) and lay a foundation for finding the plant resources rich in mangiferin. Methods: Kromasil 100C18 (4.6 mm ”250 mm, 5 μm) column was used with isocratic mobile phase of acetonitrile-0.5% phosphoric acid solution (15:85) for 10 min with a flow rate of 1 mL · min- 1 at a detection wavelength of 258 nm and a column temperature of 30 ° C.Results: Under the chromatographic conditions, the content of mangiferin and other components in the sample were well separated and the linearity was good in the range of 0.116 ~ 580 μg.mL-1. 97.5% (Polygala tenuifolia), 97.1% (Polygalaceae), 98.5% (Polygalaceae) respectively; RSD was 1.6%, 2.1%, 1.8% 0.21% .Conclusion: The method is simple, rapid, accurate, reproducible and can be used to determine the content of mangiferin in plants of different origins,