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目的 在大鼠实验性肝癌发生过程中原位显示端粒酶及其变化。方法 用含 0 .0 5 % 3′ 甲基 4 二甲基氨基偶氮苯的玉米粉喂饲大鼠 12周以制备肝癌模型。组织冰冻切片酶催化端粒延伸 PCR(原位TRAP)法检测端粒酶活性。结果 正常肝细胞、胆管细胞以及汇管区细胞一般均阴性。肝纤维化、早期肝硬化病变中一小部分肝细胞呈弱阳性 ,间质内增生的肌纤维母细胞端粒酶活性较强 ,增生的胆管细胞和结节状增生肝细胞显示弱至中等强度酶活性。不典型增生的胆管细胞及肝细胞内可见较强的酶活性。肝细胞性肝癌和胆管细胞性肝癌中绝大部分癌细胞可见端粒酶表达 ,但强弱不一。上述端粒酶活性一般位于细胞核内。结论 大鼠肝癌发生过程中端粒酶活性逐渐增加提示该酶对肿瘤细胞获得永生化特性具有重要意义。
Objective To display telomerase and its changes in situ during the development of experimental liver cancer in rats. Methods Rats were fed with corn flour containing 0.05% 3’ methyl 4 dimethylaminoazobenzene for 12 weeks to prepare a liver cancer model. Tissue frozen-section enzyme-catalyzed telomere extension PCR (in situ TRAP) assay detects telomerase activity. Results Normal liver cells, bile duct cells, and portal cell cells were generally negative. A small fraction of liver cells in liver fibrosis and early cirrhosis were weakly positive. The telomerase activity in myofibroblasts in interstitial hyperplasia was strong, and the proliferating cholangiocytic cells and nodular hyperplasia showed weak to moderately strong enzymes. active. Atypical hyperplasia of bile duct cells and hepatocytes showed strong enzyme activity. The expression of telomerase was observed in most of the cancer cells in hepatocellular carcinoma and cholangiocarcinoma, but the difference was not significant. The above telomerase activity is generally located in the nucleus. Conclusion The gradual increase of telomerase activity during the development of rat liver cancer suggests that the enzyme has important significance for the immortalization of tumor cells.