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目的:建立测定酸枣仁皂苷A和B的新方法。方法:采用胶束电动毛细管色谱法,熔硅毛细管尺寸60cm×75μm(有效柱长50cm);缓冲液组成为20mmol·L-1硼酸盐、60mmol·L-1胆酸钠和20%甲醇,pH8.2;检测波长203nm;重力进样高度22cm,进样时间7s;分离电压15kV;柱温25°C。结果:酸枣仁皂苷A和B的线性动态范围分别为30-900mg·L-1和40-1250mg·L-1(r2为0.9979、0.9969);检出限为5.68和11.86mg·L-1;900和1250mg·L-1时的精密度为0.46%和0.58%;平均加标回收率为98.1%、98.4%。结论:本方法可满足酸枣仁中皂苷A和B的测定要求,可作为酸枣仁药材的质量控制方法。
Objective: To establish a new method for the determination of saponin A and B. METHODS: Micellar electrokinetic capillary chromatography was used. The size of the fused silica capillary was 60cm×75μm (effective column length 50cm). The buffer composition was 20mmol·L-1 borate, 60mmol·L-1 sodium cholate and 20% methanol. pH8.2; Detection wavelength 203nm; Gravity injection height 22cm, injection time 7s; Separation voltage 15kV; Column temperature 25°C. Results: The linear dynamic ranges of saponin A and B were 30-900 mg·L-1 and 40-1250 mg·L-1, respectively (r2 was 0.9979 and 0.9969). The detection limits were 5.68 and 11.86 mg·L-1, respectively. The precision at 900 and 1250 mg·L-1 was 0.46% and 0.58%, respectively. The average recoveries were 98.1% and 98.4%. Conclusion: This method can meet the determination requirements of saponins A and B in Semen Ziziphi Spinosae, and it can be used as a quality control method for Suanzaoren.