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目的比较不同方式大剂量补充硫酸亚铁对大鼠骨髓造血机能的影响。方法刚断乳的雌性 Wistar 大鼠,用铁缺乏饲料喂养至血红蛋白低于100g/L。按血红蛋白含量和体重分为铁缺乏对照组、每日小剂量补铁组、每周1次小剂量补铁组、每日大剂量补铁组和每周1次大剂量补铁组。补充期12周。实验结束时,取股骨骨髓,涂片染色,观察骨髓细胞形态学和骨髓可染色铁的状况。结果对照组大鼠骨髓细胞外可染色铁减少或缺失十分明显,细胞内铁主要以0型铁颗粒幼红细胞为主。各补充组大鼠骨髓中幼红细胞增生较明显,细胞外铁多数为3型以上,铁粒幼细胞的比例超过30%。从骨髓细胞增生分级看,所有大鼠骨髓细胞增生程度表现为增生活跃或增生明显活跃,属于正常骨髓的表现形式,没有其他诸如白血病等的表现。结论 4种补充方式大鼠的铁营养状况均得到显著改善;每日大剂量补充或每周1次大剂量补充3个月,并未发现有损骨髓的造血机能。
Objective To compare the effects of different doses of high-dose ferrous sulfate on the hematopoietic function of rat bone marrow. Methods Weanling female Wistar rats were fed an iron-deficient diet until hemoglobin was below 100 g / L. According to hemoglobin content and body weight divided into iron deficiency control group, daily low-dose iron supplementation group, weekly low-dose iron supplementation group, daily high-dose iron supplementation group and weekly high-dose iron supplementation group. Supplementary period of 12 weeks. At the end of the experiment, the femur bone marrow and smear were stained to observe the morphology of bone marrow cells and the condition of bone-stainable iron. Results In the control group, the decrease or absence of stainable iron in bone marrow cells was obvious. The intracellular iron mainly consisted of 0 type iron particles. The erythrocyte proliferation in the bone marrow of each supplement group was more obvious. The majority of extracellular iron was more than type 3, and the proportion of iron granules was more than 30%. From the perspective of proliferation and differentiation of bone marrow cells, the degree of bone marrow hyperplasia in all rats showed hyperplasia or hyperplasia obviously, belonging to the manifestation of normal bone marrow without any other manifestations such as leukemia. CONCLUSION: The iron supplementation of rats in four different supplementation ways has been significantly improved. High-dose daily supplementation or high-dose once a week for 3 months did not show any effect on the hematopoietic function of bone marrow.