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目的 研究一氧化氮 (NO)合酶抑制剂L -NG -硝基精氨酸甲酯 (L -NAME)在多巴胺对小鼠胃肠推进运动影响中的作用。方法 将 32只小鼠随机分为 3组 :对照组 (n =6 ) ,静脉注射生理盐水 ;A组 (n =7)静脉注射多巴胺 ;B组 (n =19)静脉分别注射不同剂量的L -NAME ,10min后分别静脉注射多巴胺。采用含 12 %活性炭和4%黄芪胶的炭末胶液灌胃 ,计算 2 0min炭末胶液在小肠内推进距离占小肠全长的百分比 ,以此作为小肠推进速度的指标 ,同时测定灌胃 2 0min后胃内炭末胶液剩余的容积。结果 静脉单独注射多巴胺组与正常对照组相比小肠推进速度减慢 18.0 % ,胃内剩余量增加 85 .2 % ,均有显著性差异 (P <0 .0 1,P <0 .0 5 ) ;静脉预先注射L -NAME 3mg kg可以完全翻转随后给予多巴胺对胃肠推进运动的抑制作用 ;而静脉注射L -NAME(6mg kg和 12mg kg) +多巴胺组 ,与单独注射多巴胺组相比 ,小肠推进速度分别减慢 3.8%和 18.8% ,而胃内剩余量分别增加2 8.8%和 45 .6 % (P <0 .0 5 )。结论 静脉注射多巴胺对小肠推进速度的影响除直接作用于小肠平滑肌细胞外 ,还与胃排空速度有关 ;NO合酶抑制剂通过调节胃排空过程影响小肠推进运动
Objective To investigate the effect of nitric oxide (NO) synthase inhibitor L-N-N-nitro-arginine methyl ester (L-NAME) on gastrointestinal motility in mice induced by dopamine. Methods 32 mice were randomly divided into 3 groups: control group (n = 6), intravenous injection of saline; group A (n = 7) intravenous injection of dopamine; group B -NAME, 10min after intravenous injection of dopamine. Adopting carbon powder containing 12% activated carbon and 4% tragacanth gum for intragastric administration, the percentage of the propulsion distance in the small intestine to the total length of the small intestine in 20 min was calculated as the index of the intestinal propulsion speed and the intragastric 2 0min after the end of the stomach charcoal glue the remaining volume. Results Compared with the normal control group, the intestinal propulsion speed slowed down by 18.0% and the residual volume in the stomach increased by 85.2% compared with the normal control group (all P <0.01, P <0.05) ; Intravenous injection of L-NAME 3 mg kg completely reversed the inhibitory effect of dopamine on gastrointestinal propulsive movement; whereas intravenous injection of L-NAME (6 mg kg and 12 mg kg) + dopamine group, compared with the dopamine group alone, The propulsion rate slowed by 3.8% and 18.8%, respectively, while the intragastric remnant increased by 8.88% and 45.6%, respectively (P <0.05). Conclusion The effect of intravenous dopamine on small intestine propulsion velocity is related to the rate of gastric emptying except directly acting on the small intestinal smooth muscle cells. NO synthase inhibitor affects small intestine propulsion through regulating gastric emptying