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环孢菌素A(Cyclosporin A,Cy A)作为一种新型的免疫抑制剂已成功的用于器官移植,此外它对胰岛素依赖性糖尿病以及一些其他疾病也有较好的疗效。CyA的研究过程中,需要测定发酵液和提取分离各步骤样品中CyA的含量。由于样品中含CyA、B、C、D等多种组份,各组份生物活性不同,因而生物测定法不适用。HPLC测定CyA的发酵液和成品已有文献报道。本文报道我们根据前人工作所作的改进。用Beckman 344型高效液相色谱仪,色层柱为Shim-Pack CLC-C_8(6mm×150mm)。柱温67℃,移动相为乙腈:磷酸水溶液(pH2.5)(65∶35),流速2ml/分,以165型紫外检测器,采用214um(使用色谱纯乙腈移动相时)或226nm(用分析纯乙腈)。检测器灵敏度为0.100AUFS。进样量、标准品与样
As a new immunosuppressant, Cyclosporin A (Cy A) has been successfully used in organ transplantation. In addition, it also has a good effect on insulin-dependent diabetes mellitus and some other diseases. CyA research process, the need for determination of fermentation broth and extraction and separation steps of the sample CyA content. As the sample contains CyA, B, C, D and other components, the biological activity of different components, so the bioassay does not apply. HPLC determination of CyA fermentation broth and finished products have been reported in the literature. This article covers the improvements we make based on previous work. The column was Shim-Pack CLC-C_8 (6 mm × 150 mm) using a Beckman 344 high performance liquid chromatograph. The column temperature was 67 ° C and the mobile phase consisted of acetonitrile: phosphoric acid (pH 2.5) (65:35) at a flow rate of 2 ml / min using a 165 um detector using 214 um Analytical pure acetonitrile). Detector sensitivity is 0.100AUFS. Injection volume, standard and sample