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【目的】肠道细菌桃色欧文氏菌Erwinia persicina是小菜蛾Plutella xylostella幼虫肠道的优势细菌,本研究旨在阐明桃色欧文氏菌的代谢表型特征。【方法】采用BIOLOG细胞表型芯片技术系统研究了桃色欧文氏菌的细胞表型;采用PM 1-10代谢板,对桃色欧文氏菌的950种代谢表型进行了测定。【结果】桃色欧文氏菌能代谢39.47%的碳源、89.74%的氮源、100%的硫源和100%的磷源;高效代谢的碳源为有机酸类和碳水化合物类,高效代谢的氮源为氨基酸类。该肠道细菌表现出261种不同的氮源代谢通路和95种生物合成通路。桃色欧文氏菌具有广泛的适应性,能在分别具有高达9%氯化钠、4%氯化钾、5%硫酸钠、20%乙二醇、6%甲酸钠、2%尿素、6%乳酸钠、200mmol/L磷酸钠(p H 7.0)、20 mmol/L苯甲酸钠(p H 5.2)、100 mmol/L硫酸铵(p H 8.0)、100 mmol/L硝酸钠和100 mmol/L亚硝酸钠的渗透溶液中正常代谢;其适应p H值范围为4.5~10,最适7.0。在多种氨基酸的作用下,桃色欧文氏菌均表现出脱羧酶和脱氨酶活性。【结论】桃色欧文氏菌的代谢特征增加了我们对该肠道细菌,特别是其与宿主昆虫的互作及其在肠道环境中的适应性的认识,同时表明通过降低桃色欧文氏菌密度来防治小菜蛾的可能性。
【Objective】 Erwinia persicina, an intestinal bacteria, is the dominant bacterium in the intestinal tract of Plutella xylostella larvae. The purpose of this study is to elucidate the metabolic phenotype of Erwinia peach. 【Method】 The cell phenotype of Erwinia amylovora was studied systematically by BIOLOG cell phenotyping chip technique. 950 metabolic phenotypes of Erwinia amylovora were determined by using PM 1-10 metabolic plates. 【Result】 Erwinia peachiensis could metabolize 39.47% carbon source, 89.74% nitrogen source, 100% sulfur source and 100% phosphorus source. The highly efficient carbon sources were organic acids and carbohydrates, which were metabolized efficiently Nitrogen sources are amino acids. The gut bacteria showed 261 different nitrogen source pathways and 95 biosynthetic pathways. Erwinia & lt; RTI ID = 0.0 & gt; peachii & lt; / RTI & gt; has a wide range of applicability and can be used in cultures with up to 9% sodium chloride, 4% potassium chloride, 5% sodium sulfate, 20% ethylene glycol, 6% sodium formate, 200 mmol / L sodium phosphate (p H 7.0), 20 mmol / L sodium benzoate (p H 5.2), 100 mmol / L ammonium sulfate (p H 8.0), 100 mmol / L sodium nitrate and 100 mmol / L sodium nitrite Osmotic solution normal metabolism; its adaptation p H range of 4.5 to 10, the best 7.0. Under the action of many kinds of amino acids, Erwinia peachiensis showed decarboxylase and deaminase activities. 【Conclusion】 The metabolic characteristics of Erwinia amylovora increased our knowledge of the interaction between the intestinal bacteria, especially their host insects and their adaptability in the intestinal environment, and at the same time showed that by reducing the density of Erwinia amylovora To control the possibility of diamondback moths.