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目的:观察决明方对肥胖大鼠非酒精性脂肪肝的干预作用及对肝脏固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响。方法:雄性SD大鼠随即分为对照组普通饲料喂养,模型组和决明方组高脂饲料喂养制备肥胖并发脂肪肝模型。8周后决明方组用决明方口服液治疗,模型组和对照组给予等量注射用水,持续4周。检测血脂、肝组织三酰甘油(TG)和肝功能,观察肝组织病理学改变,RT-PCR测定肝SREBP-1c及其靶基因脂肪酸合成酶(FAS)mRNA表达,Western Blot测定肝SREBP-1蛋白表达。结果:与模型组比较,决明方组肝TG和血清TG明显降低(P<0.01,P<0.05);血HDL-C明显升高(P<0.01);肝脂肪变性明显减轻;肝SREBP-1c及FAS的mRNA表达均明显下降(P<0.01);SREBP-1蛋白表达明显减少(P<0.01)。结论:决明方下调SREBP-1c及其靶基因的表达,对非酒精性脂肪肝有一定的治疗作用。
OBJECTIVE: To observe the effect of JXJM on non-alcoholic fatty liver in obese rats and its effect on the expression of hepatic steroid regulatory element-binding protein-1c (SREBP-1c). Methods: Male Sprague-Dawley rats were randomly divided into control group fed normal diet, model group and Cassia group treated with high-fat diet to prepare obese and complicated fatty liver model. After 8 weeks, Cassia Decoction was treated with Dingming Fang Oral Liquid, and the model group and the control group were given the same amount of water for 4 weeks. Serum lipids, triglyceride (TG) and liver function of liver tissue were observed. Pathological changes of liver tissue were observed. MRNA expression of SREBP-1c and its target gene FAS were detected by RT-PCR. Western Blot was used to detect liver SREBP-1 Protein. Results: Compared with the model group, the liver TG and serum TG in the Decoction group were significantly decreased (P <0.01, P <0.05); the HDL-C in serum was significantly increased (P <0.01); the hepatic steatosis was significantly reduced; 1c and FAS mRNA (all P <0.01). The expression of SREBP-1 protein was significantly decreased (P <0.01). Conclusion: Cassia Decoction down-regulates the expression of SREBP-1c and its target genes and has a certain therapeutic effect on non-alcoholic fatty liver.