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目的调查我国东北林区蜱标本中嗜吞噬细胞无形体的感染情况。方法2010-2011年在东北林区采集蜱标本,用聚合酶链反应方法扩增gltA基因片段,分析东北林区蜱中无形体的感染情况,代表阳性片段联用长片段16S rRNA进行扩增测序确认;并将所得序列与GenBank中已注册的序列进行比对分析。结果共检测蜱2293只,其中2010年1161只,阳性率4.91%,2011年1132只,阳性率4.24%,2年的感染率差异无统计学意义(χ2=0.373,P=0.830;χ2=1.789,P=0.409)。全沟硬蜱阳性率4.86%(91/1872),长角血蜱为3.41%(14/411),森林革蜱为0(0/10),3个蜱种的感染率差异无统计学意义(P>0.05)。测得的gltA序列中,NE-gltA-1与Hc346株(GenBank:GU935788)最相似,仅差2 bp,相似性99%;NE-gltA-2与MDJ-Ip92株(HQ396224)序列一致,相似性100%。与2个序列相似性最远的是ZJ-HGA-72株(DQ458811),相差53 bp,相似性84%。测得的16S rRNA序列与我国从东北鼠源分离株China-C-T(tGQ412339)最接近,相似性为99%,与Florida株(AF309867)相似性最远,相似性97%。结论我国东北林区蜱中普遍存在嗜吞噬细胞无形体的感染,全沟硬蜱和长角血蜱可能为无形体的媒介宿主。
Objective To investigate the infection of phagocytic phagocytes in the ticks of the forest in Northeast China. Methods Totally collected ticks from 2010 to 2011 in the forest area of northeastern China were amplified by polymerase chain reaction. The gltA gene fragment was amplified by polymerase chain reaction (PCR), and the infection of the ticks in the forest was analyzed. The positive fragments were sequenced and amplified by 16S rRNA Confirmed; and the resulting sequence with the registered GenBank sequence analysis. Results A total of 2293 ticks were detected, of which 1161 were positive in 2010, with a positive rate of 4.91%. In 2011, 1132 were positive, with a positive rate of 4.24%. There was no significant difference in infection rates at 2 years (χ2 = 0.373, P = 0.830; , P = 0.409). The positive rate of all ticks was 4.86% (91/1872), the percentage of H. longicornis was 3.41% (14/411), and the number of forest ticks was 0 (0/10). The infection rates of three ticks showed no significant difference (P> 0.05). The sequence of gltA-1 was the most similar to that of Hc346 (GenBank: GU935788), with a difference of 2 bp and a similarity of 99%. The sequence of NE-gltA-2 and MDJ-Ip92 (HQ396224) Sex 100%. The most similar to the two sequences was ZJ-HGA-72 (DQ458811), a difference of 53 bp and a similarity of 84%. The 16S rRNA sequence was closest to China from the Northeast China isolate C-T (tGQ412339), and the similarity was 99%. The similarity was 97% with Florida strain (AF309867). Conclusion Infection of phagocytic phagocytes in ticks in forest areas of northeastern China is widespread, and Ixodes versicolor and C. longicornis may be vector hosts for the inorganism.