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目的：建立高效液相色谱法检测花生油中黄曲霉毒素（aflatoxin，AFT）的分析方法。方法：试样经甲醇- 水溶液（70:30，V:V）提取，免疫亲和柱净化，通过高效液相色谱法测定试样中的黄曲霉毒素。结果：黄曲霉毒素B1、B2、G1、G2分别在4.874～38.988、1.323～10.584、4.649～37.188 及1.449～11.592μg/L 浓度范围内，与其峰面积呈良好线性关系，相关系数r 均大于0.999；加标回收率为84.5%～106%，相对标准偏差（RSD）为2.3～4.8%。结论：该方法准确、灵敏度高，适用于花生油中黄曲霉毒素的检测。“,”Objective: To establish a method for the determination of aflatoxins (AFT) in peanut oil by high performance liquid chromatography. Methods: The samples were extracted by 70% methanol aqueous solution and the extracts were purified by immunoaffinity column, and then detected by high performance liquid chromatography. Results: Aflatoxins showed good linear relationship with their chromatographic peaks area in the concentration range of 4.874～ 38.988 μg/L (AFT B1), 1.323 ～ 10.584 μg/L (AFT B2), 4.649 ～ 37.188 μg/L (AFT G1) and 1.449 ～ 11.592 μg/L (AFT G1). The correlation coefficients (r) were all above 0.999.The recovery rate were 84.5% ～ 106%, and the relative standard deviation (RSD) were 2.3 ～ 4.8%. Conclusion: The method which has high accuracy and high sensitivity is suitable for the detection of aflatoxins in peanut oil.