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目前所用的百日咳菌苗(BPV)均含有脂多糖(LPS),LPS可引起不良反应。本文介绍一种用去污剂Emulphogene BC720从无细胞百日咳菌苗中去除LPS的方法。试验采用Ⅰ相百日咳杆菌,在Cohen-Wheeler琼脂培养基中经36℃培养96小时,收获的菌体用磷酸盐缓冲液(PBS)洗涤,再将该培养物加至含有0.02%硫柳汞、0.75MNaCl、0.05M磷酸钠缓冲液(pH7.0)中,置4℃过夜灭活后抽提各组分。离心去除菌体,上清中加入20%饱和度硫酸按,置4℃作用30分
Currently used pertussis vaccine (BPV) contain lipopolysaccharide (LPS), LPS can cause adverse reactions. This article describes a method for removing LPS from Acellular pertussis vaccine using the detergent Emulphogene BC720. The test was performed on Bordetella pertussis (Phase I) and cultured in a Cohen-Wheeler agar at 36 ° C for 96 hours. The harvested cells were washed with phosphate buffered saline (PBS) and added to a solution containing 0.02% thimerosal, 0.75M NaCl , 0.05 M sodium phosphate buffer (pH 7.0), and the components were extracted after being inactivated overnight at 4 ° C. Remove the cells by centrifugation, the supernatant by adding 20% sulfuric acid press, set at 4 ° C for 30 minutes