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[目的]探讨AE1/AE3免疫组化标记检测食管癌根治标本中淋巴结内微小转移癌及食管周围软组织癌浸润的意义。[方法]对30例食管癌根治切除后阴性的淋巴结及食管周软组织的石蜡包埋组织用SP法进行AE1/AE3标记。[结果]30例中337个淋巴结中292个HE切片诊断为阴性淋巴结者行AE1/AE3标记,发现23个阳性淋巴结,阳性表达率为7.9%。根据HE染色阴性再经AE1/AE3标记发现食管周软组织内微小癌灶者,其中黏膜下癌为0/3,癌浸及肌层者为7/11(63.6%)、癌浸及纤维膜者为13/16(81.3%)。[结论]AE1/AE3标记可为食管癌淋巴结的清扫提供参考,为判断癌细胞的浸润深度及范围、PTNM分期提供依据。
[Objective] To investigate the significance of AE1 / AE3 immunohistochemical staining for the detection of micrometastases in lymph nodes and infiltration of soft tissue carcinoma of the esophagus in radical specimens of esophageal cancer. [Method] Thirty paraffin-embedded tissues of negative esophageal lymph nodes and esophageal soft tissues after radical resection of esophageal cancer were stained with AE1 / AE3 by SP method. [Results] 292 HE sections of 337 lymph nodes in 30 cases were diagnosed as negative lymph nodes by AE1 / AE3 markers, and 23 positive lymph nodes were found, the positive rate was 7.9%. According to HE staining negative and AE1 / AE3 marker found in esophageal and soft tissue within the small cancer lesions, including sub-subcutaneous cancer was 0/3, cancer and myomectomy was 7/11 (63.6%), cancer and fiber membrane immersion For 13/16 (81.3%). [Conclusion] The AE1 / AE3 marker can provide a reference for the dissection of lymph node in esophageal cancer. It provides a basis for determining the depth and extent of cancer invasion and PTNM staging.