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目的研究核转录因子(E2F-1)和原癌基因(c-Myc)在年龄在35岁以下宫颈上皮内瘤病变(CIN)和宫颈鳞癌(SCC)中的表达及其相关性,探讨E2F-1和c-Myc在SCC发生发展中的关系与临床病理关系。方法选择手术切除的SCC组织标本40份、CIN标本31份,正常宫颈组织(NCE)10份,采用免疫组化SP法检测各组E2F-1和c-Myc的表达情况,并通过统计学方法分析其表达情况以及与肿瘤分期、病理分型、淋巴结转移等之间的相互关系。结果 SCC组中E2F-1阳性表达率为77.5%(31/40),显著高于CIN组和NCE组(P<0.01),E2F-1的表达与SCC的分期及淋巴结转移有相关性(P<0.01)。c-Myc在SCC中的阳性表达率为62.5%(25/40),显著高于CIN组和NCE组(P<0.01),c-Myc在SCC中的表达与分化及淋巴结转移有关(P<0.01),但其与分期无关。通过相关性分析发现两者在SCC组织中表达呈正相关。结论 E2F-1和c-Myc在SCC发生、发展中起重要作用,E2F-1和c-Myc上调表达可作为SCC预后的判断指标。
Objective To investigate the expression and correlation of nuclear transcription factor (E2F-1) and protooncogene (c-Myc) in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (SCC) -1 and c-Myc in the development and progression of SCC and clinicopathological relationship. Methods 40 specimens of SCC, 31 specimens of CIN and 10 specimens of normal cervical tissue were collected. The expression of E2F-1 and c-Myc in each group were detected by immunohistochemical SP method. Analyze its expression and the correlation with tumor staging, pathological type, lymph node metastasis and so on. Results The positive rate of E2F-1 in SCC group was 77.5% (31/40), significantly higher than that in CIN group and NCE group (P <0.01). The expression of E2F-1 was correlated with the stage of SCC and lymph node metastasis <0.01). The positive expression rate of c-Myc in SCC was 62.5% (25/40), which was significantly higher than that in CIN group and NCE group (P <0.01). The expression of c-Myc in SCC was correlated with differentiation and lymph node metastasis (P < 0.01), but it has nothing to do with staging. Correlation analysis showed that there was a positive correlation between the two expressions in SCC tissues. Conclusions E2F-1 and c-Myc play an important role in the occurrence and development of SCC. Up-regulated expression of E2F-1 and c-Myc can be used as a prognostic indicator of SCC.