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棉大卷叶螟是棉花生长中后期的重要食叶性害虫.本研究以浸叶法测定低浓度(0.5μg·g~(-1))的CrylAc毒蛋白处理对棉大卷叶螟连续3个世代生长发育与种群增长的影响.结果表明:处理后的棉大卷叶螟幼虫期比对照延长1.78~2.00 d,成虫寿命延长1.44~1.94 d,蛹体质量增加5.3%~11.8%;3个世代的单雌产卵量分别增加65.1%、47.3%和37.6%,种群趋势指数分别提高11.4%、17.5%和20.0%.这些指标与对照均存在显著差异.蛹历期、羽化率、性比和卵孵化率等与对照无显著差异.研究表明,转Bt基因棉长期种植后棉大卷叶螟的种群数量将会增加,需要进一步评估转基因棉种植的风险性,加强转基因棉田害虫的综合治理.
Cotton leaf roller is an important leaf-eating pest in the mid-late cotton growth stage.In this study, leaf-opening method was used to determine the effect of CrylAc at a low concentration (0.5 μg · g -1) The results showed that the larval duration of the treated leafroller larvae was 1.78 ~ 2.00 d longer than that of the control, and the longevity of adult larvae was 1.44-1.94 d, with the pupal mass increasing 5.3% ~ 11.8% .3 The single fecundity of females increased by 65.1%, 47.3% and 37.6%, respectively, and the population trend index increased by 11.4%, 17.5% and 20.0%, respectively.These indices were significantly different from the control Ratio and hatching rate of eggs and other no significant difference with the control study shows that transgenic Bt cotton long-term planting cotton leaf roller population will increase, the need to further assess the risk of transgenic cotton planting, strengthen the synthesis of transgenic cotton pests Governance.