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Objective:To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer.Methods:Plasmids pcDNA3-hLRH-1 were introduced into SW480 ceils via lipofectamine.The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting.respectively.MTT assay was carried out to survey the proliferation of SW80 cells with overexpression of hLRH-1.Meanwhile.the expression of proliferation-related genes cyclin E1 and cyclin D1.and apoptosis-related genes PTEN and Rb1.were analyzed by realtime RT-PCR.Result:The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1.The expression of cyclin E1 was up-regulated significantly,while that of PTEN and Rb1 were down-regulated jn SW480 cells with overexpressed hLRH-1.Conclusion:The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1.as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1.