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研究Ｃ５７小鼠出生后视网膜的发育和增殖细胞核抗原（ＰＣＮＡ）表达、两者关系以及细胞动力学过程。方法采用ＨＥ染色及免疫组化标记ＰＣＮＡ的方法，对出生后第１～２１天的Ｃ５７小鼠视网膜神经层的细胞发育作连续性观察。结果光镜下观察到，Ｃ５７小鼠出生后，视网膜神经层仍然有一个发育过程。神经节细胞层在出生时即已经形成；外丛状层在出生后第６天才分化出来，而内、外核层细胞形态上的接近成熟小鼠的形态，分别在出生后第１０天、第１９天。ＰＣＮＡ显示在外层神经细胞层和节细胞各有一次和增殖相关的阳性表达。３个细胞层在形态学的分化基本完成后，又出现了一次结束时间是由外向内变化的阳性过程（外核层：第１１～１５天；内核层：第８～１６天；神经节细胞层：第１６～２１天），这一次阳性表达过程可见到细胞形态向成年形态的转化。结论Ｃ５７小鼠出生后视网膜神经层的发育，分为细胞分化和细胞成熟两个阶段，分化阶段的细胞顺序是以细胞空间位置决定的，成熟阶段的顺序是按神经传导的方向发展的，可能与细胞的功能相关。ＰＣＮＡ的表达和细胞的增殖、分化、成熟在时间、空间上具有高度的相关性。
To study the retina development and the expression of proliferating cell nuclear antigen (PCNA) in C57 mice after birth, the relationship between them and the process of cell dynamics. Methods HE staining and immunohistochemical staining of PCNA were used to observe the cell development of retinal neurons in C57 mice from the first day to the 21st day after birth. Results Under light microscope, there was still a developmental process of retinal neurons after C57 mice were born. The ganglion cell layer was formed at birth; the outer plexiform layer differentiated only on the 6th day after birth, and the morphology of the inner and outer nuclear layer cells was similar to that of the mature mouse. On the 10th day after birth, 19 days. PCNA showed a positive proliferative-related positive expression in the outer layer of nerve cells and node cells. After the morphological differentiation of the three cell layers was basically completed, an end-time positive change from the extrinsic end to the end was observed (outer nuclear layer: days 11 to 15; inner nuclear layer: days 8 to 16; ganglion cells Layers: Day 16-21), this time positive expression can be seen from the cell morphology to adult morphology. Conclusion The development of retinal neurons in postnatal C57 mice is divided into two stages: cell differentiation and cell maturation. The order of the cells in the differentiation stage is determined by the spatial location of the cells. The order of the maturation stage is in the direction of nerve conduction. Related to cell function. PCNA expression and cell proliferation, differentiation, maturation in time and space with a high degree of correlation.