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目的:观察暴露于十溴联苯醚(brominated diphenyl ethers-209,BDE-209)的子鼠脑组织发育的情况,电镜下观察子鼠突触超微结构及数量的变化。方法:经胃灌法建立母源性BDE-209动物模型(300mg/kg.d-1,以精制花生油溶解)。实验组(A):自购买日始母鼠胃灌BDE-209直至生育第一代子鼠断乳;实验组(B):自购买日始胃灌单纯花生油至妊娠,妊娠后改为胃灌BDE-209直至生育第一代子鼠断乳;实验组(C):自购买日始胃灌单纯花生油至分娩新一代子鼠,仅在哺乳期胃灌BDE-209;对照组(D):自购买日始一直胃灌单纯等量花生油。每组选取子代鼠作为研究对象,制作组织标本及电镜标本;尼氏染色光镜下观察子鼠脑组织的形态学变化;透射电镜下观察子鼠脑组织的结构及突触结构和数量的变化。结果:(1)经尼氏染色,子鼠成年后的脑组织形态学变化不明显,未发现特殊结构的差异;(2)电镜下见实验组子鼠脑细胞及突触超微结构发生变化;(3)突触密度计数:各组大鼠子代海马CA3区分子层内突触密度计数(个/70μm2)结果显示,在母鼠接触BDE-209的过程中,A组与B、C、D组比较,差异均有显著统计学意义(P<0.05)。B、C组与对照组比较无显著差异,B组与C组之间差异无统计学意义(P>0.05)。结论:母鼠接触BDE-209可以导致子鼠脑神经细胞及突触超微结构发生改变,影响子鼠突触的形成发育,降低突触数量。
OBJECTIVE: To observe the development of rat brain tissues exposed to brominated diphenyl ethers-209 (BDE-209) in vitro and to observe the changes of ultrastructure and number of synapses in mice. Methods: Animal models of maternal BDE-209 (300 mg / kg.d-1, dissolved in refined peanut oil) were established by gastric perfusion. The experimental group (A): from the date of purchase, the mother rats stomach perfusion of BDE-209 until the first generation of offspring offspring; experimental group (B): since the day of purchase, simple gastric perfusion of peanut oil to pregnancy, BDE-209 was weaned until the first generation of offspring. The experimental group (C): BDE-209 was infused only from lactation stage to the new generation of offspring, Since the date of purchase has been gastric perfusion pure peanut oil. Each group selected offspring mice as research object, making tissue samples and electron microscopy specimens; Observing morphological changes of brain tissue in neonatal rats under Nissl staining; Observing the structure and synapse structure and number of brain tissue in transmission electron microscope Variety. Results: (1) The morphological changes of brain tissue were not obvious after Nissei staining, and no special structure was found. (2) The changes of brain cells and synaptic ultrastructure in experimental mice were observed under electron microscope ; (3) Synaptic density count: The number of synaptic density in the CA3 subfields of each rat offsprings (A / 70μm2) showed that in the process of female rats contacting BDE-209, A group and B, C , D group, the difference was statistically significant (P <0.05). There was no significant difference between group B and group C and control group, but there was no significant difference between group B and group C (P> 0.05). CONCLUSION: Exposure of BDE-209 to maternal mice can result in changes of neuronal cells and synaptic ultrastructure in the rat brain, affecting the formation and development of synapses in mice and decreasing the number of synapses.