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目的 研究外源性 α-突触核蛋白(α-syn)及其A53T突变型对星形胶质细胞的激活作用.方法 分离培养小鼠原代星形胶质细胞,经 α-syn和A53T刺激后,用实时荧光定量聚合酶链反应和Western blot检测Toll样受体(TLRs)和核转录因子-κB(NF-κB)的表达变化.结果 与对照组比较,2种 α-syn提高TLRs1~4和NF-κB的表达水平(P<0.05),其中A53T作用更强;Western blot检测结果表明,A53T提高TLR2和NF-κB的蛋白水平.结论 α-syn激活星形胶质细胞介导的固有免疫反应,为神经退行性疾病机制提供免疫学证据.“,”Objective To investigate the activation effect of α-synuclein (α-syn) on astrocytes and innate immunity. Methods Primary astrocytes of mice were isolated and treated with α-syn (wild type, Wt) and its A53T mutant. Quantitative real-time PCR (qRT-PCR) and Western blot analysis were used to examine altered expressions of toll-like receptors (TLRs) 1-4 and NF-κB. Results In comparison with the control groups, Wt α-syn and its A53T mutant increased mRNA expressions of TLRs 1-4 and NF-κB, displaying a statistical significance (P < 0.05), with the A53T mutant having more potent immune induction effect than Wt α-syn. Western blot analysis showed the A53T mutant upregulated protein expressions of TLR2 and NF-κB. Conclusions Alpha synuclein is a powerful immunogenic substance to activate the astrocyte-mediated innate immune response, which provides a strong immunological evidence for the pathogenesis of neurodegenerative diseases.