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申请公布号:CN106198830A申请公布日:2016.12.07申请人:泰山医学院摘要本发明提供一种液相色谱检测三单体的方法,使用FL2200液相色谱仪,N2000色谱工作站,色谱柱Kromstar C18规格为150 mm×4.6 mm,5μm;流动相a与流动相b的体积比为80~90∶10~20;检测波长:220 nm;流速:0.8 m L/min;进样体积:20μL;柱温:30℃。使用本发明的检测方法,检测时间小于10 min;分析精度可达0.01 ng;重现性好,相对误差为0.2%;操作简易、分析全部自动化;达到对间苯二甲
Application Publication Number: CN106198830A Published Date: 2016.12.07 Applicant: 泰山 医学院 Abstract The present invention provides a liquid chromatography method for the detection of three monomers using a FL2200 liquid chromatograph, a N2000 chromatography workstation, a Kromstar C18 column Was 150 mm × 4.6 mm, 5 μm; the volume ratio of mobile phase a to mobile phase b was 80-90: 10-20; the detection wavelength was 220 nm; the flow rate was 0.8 m L / min; the injection volume was 20 μL; : 30 ° C. Using the detection method of the invention, the detection time is less than 10 minutes; the analysis precision can reach 0.01 ng; the reproducibility is good with the relative error of 0.2%; the operation is simple and the analysis is fully automated;