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本文向读者介绍一种以廉价的、可重复使用的容器,用表面接菌技术对三龄一点粘虫(Pseudaletia unipuncta)施用杀蛾多形孢虫(Nosema necatrix)进行生物测定的方法。每一生测单元的结构是:在一只盛有半小杯凝固了的半合成人工饲料的塑料小杯里,用一只直径7mm、长25mm 的小玻管(可用指形管截去底部而成),插入人工饲料10mm,玻管的另一端用棉球塞紧。由于初龄幼虫仅能食下小杯中人工饲料总面积的极小部分,所以孢子的涂布和幼虫的取
This article introduces the reader to a method for bioassaying the application of Nosema necatrix to Pseudaletia unipuncta by surface-inoculation using an inexpensive, reusable container. The structure of each bioassay unit is: in a plastic cup filled with semi-synthetic semicircular synthetic artificial diet half a cup, with a small glass tube diameter 7mm, 25mm long (the fingers can be used to cut off the bottom and Into the artificial diet 10mm, the other end of the tube with a tampon tight. Because young larvae eat only a tiny fraction of the total artificial diet in small cups, application of spores and larvae