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[目的]探讨介孔纳米SiO2对小鼠巨噬细胞株RAW264.7细胞的毒性和氧化损伤作用。[方法]设4个浓度组(2.5、10、50、100μg/mL)和生理盐水对照组,染毒24h后,用四甲基偶氮唑盐(MTT)方法观察细胞毒性,并根据染毒后细胞的总蛋白(TP)、乳酸脱氢酶(LDH)、一氧化氮(NO)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)的变化情况分析介孔纳米SiO2的细胞毒性和氧化损伤作用。[结果]10、50和100μg/mL浓度组细胞存活率随着染毒浓度的升高而降低,差异有统计学意义;50、100μg/mL浓度组细胞及其上清液中TP、LDH、NO和MDA含量随染毒浓度增加而升高,而GSH和SOD含量降低;而且与细胞发生融解破碎、间隙加大等形态学改变情况相符。[结论]介孔纳米SiO2对体外培养巨噬细胞株RAW264.7具有细胞毒性和氧化损伤作用,且随剂量的加大而增强。
[Objective] To investigate the toxicity and oxidative damage of mesoporous nano-SiO2 on murine macrophage cell line RAW264.7. [Method] The four concentration groups (2.5, 10, 50 and 100μg / mL) and the normal saline control group were treated with MTT for 24 hours. The cytotoxicity (TP), lactate dehydrogenase (LDH), nitric oxide (NO), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) The effects of mesoporous nano-SiO2 on cytotoxicity and oxidative damage were analyzed. [Results] The cell viabilities of 10, 50 and 100 μg / mL groups decreased with the increase of exposure concentration, the difference was statistically significant. The concentrations of TP, LDH, The content of NO and MDA increased with the increase of the concentration, while the content of GSH and SOD decreased, but also with the morphological changes of the cells such as thawing and breaking, the gap increased. [Conclusion] Mesoporous nano-SiO2 has cytotoxicity and oxidative damage on macrophage cell line RAW264.7 cultured in vitro, and increases with the increase of dosage.