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目的记录电刺激诱发的前庭电位,排除各种干扰,证明此电位的确来自前庭。方法将刺激电极置于豚鼠圆窗,用0.05ms电流(0.25~1.2mA)刺激后,经头皮电极可记录到诱发电位波;分别选择性切断前庭神经、蜗神经或面神经,给予白噪声掩蔽观察诱发电位波波形变化。结果电刺激豚鼠圆窗,可远场记录到诱发电位波,标记为V1、V2、V3,潜伏期分别为(0.973±0.086)、(1.618±0.176)和(2.416±0.274)ms。该电位具有生物电反应特性,有神经兴奋阈值、饱和性、适应性、对神经完整性的依赖性。切断同侧蜗神经及面神经,在强白噪声掩蔽下,诱发电位波潜伏期无明显变化;切断同侧前庭神经和处死动物后诱发电位消失。诱发电位分别排除了蜗神经、面神经兴奋和刺激伪迹的可能,具有前庭源性,反映了单侧耳前庭功能。结论电刺激豚鼠圆窗可记录纯前庭源诱发电位。
Objective To record the electrical stimulation of the vestibular potential, to exclude all kinds of interference, to prove that this potential is indeed from the vestibule. Methods The stimulation electrodes were placed in the round window of guinea pig and stimulated by 0.05ms current (0.25 ~ 1.2mA). The evoked potential wave was recorded through the scalp electrodes. The vestibular nerve, cochlear nerve or facial nerve were selectively cut off, White noise masking was observed evoked potential wave waveform changes. Results The guinea pig round window was electrically stimulated and evoked potentials were recorded in the far field and labeled as V1, V2 and V3 with latency of (0.973 ± 0.086), (1.618 ± 0.176) and (2), respectively. 416 ± 0.274) ms. This potential has bioelectrical response properties, with nerve excitement thresholds, saturation, adaptability, and neuronal integrity dependence. Cut off the ipsilateral cochlear nerve and facial nerve, in the strong white noise masking, evoked potential wave latency did not change significantly; cut the ipsilateral vestibular nerve and sacrificed animals evoked potential disappeared. Evoked potentials exclude excitability of the cochlear nerve, facial nerve and stimulus artifact, respectively, with vestibular origin, reflecting the unilateral ear vestibular function. Conclusion Electrically stimulated guinea pig round window can record pure vestibular evoked potentials.