论文部分内容阅读
从烤烟叶际分离出园褐固氮菌(Azotobacter Chroococcum Beijerinck)、维涅兰德固氮菌(Azotobacter vinelandii Lipmann )、运动拜叶林克氏菌(Beijerin-ckia mobilis Derx)。测定了它们在叶面上的数量,其最高菌数为每平方厘米叶面7.0×10~4个,它们的固氮强度分别是每消耗1克葡萄糖固氮12.1毫克、9.8毫克和3.8毫克,固氮菌群体密度随着叶面渗出的糖量增加而上升。叶面露水糖浓度为16—123ppm,碳氮此是4.5～29:1。土壤固氮菌是叶际固氮菌的侵染源,其附生种类取决于叶际生境的理化条件。此外用多刺孢毛霉(Mucor spinescens Lendner)作指示菌测定了固氮菌的刺激作用,其中园褐固氮菌的刺激圈为3.95厘米;也测定了固氮菌对伴生菌环状芽孢杆菌(Bacillus circulans Jordar)的刺激作用,固氮菌的存在增强了环状芽孢杆菌对两株交链孢霉的拮抗作用。在幼苗水培时用固氮菌培养液接种叶面,刺激了叶片的生长。探讨了叶面施用固氮菌剂的可能性。
Azotobacter Chroococcum Beijerinck, Azotobacter vinelandii Lipmann and Beijerin-ckia mobilis Derx were isolated from flue-cured tobacco leaves. Their maximum numbers of foliage were determined to be 7.0 × 10 ~ 4 per square centimeter. Their nitrogen fixation strength was 12.1 mg, 9.8 mg and 3.8 mg respectively for 1 g glucose nitrogen fixation, Population density increased with the increase of sugar exudation on the foliage. Leaf dew sugar concentration of 16-123ppm, carbon and nitrogen this is 4.5 ~ 29: 1. Soil nitrogen-fixing bacteria is the infection source of nitrogen-fixing bacteria in leaves, the epiphytic species depend on the physical and chemical conditions of the inter-leaf habitats. In addition, Mucor spinescens Lendner was used as indicator bacteria to determine the stimulating effect of azotobacter, in which the azotobacter sphaerocephalus stimulus was 3.95 cm. The ability of azotobacter against Bacillus circulans Jordar), the presence of azotobacter enhanced the antagonism of B.ericii to two strains of Alternaria. In the seedling water culture with nitrogen fixing bacteria inoculated leaves, stimulating the growth of leaves. The possibility of foliar application of azotobacter was discussed.