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目的:考察吴茱萸对左金丸中盐酸小檗碱、盐酸巴马汀和盐酸药根碱在大鼠组织中分布动力学的影响,探讨吴茱萸的引经作用机制。方法:大鼠分别灌胃给予黄连、黄连吴茱萸合煎液,于给药后不同时间点采集组织样本,利用UPLCMS/MS测定组织中3种黄连生物碱类成分的经时变化,采用DAS 2.0软件计算组织分布动力学参数、相对摄取率(Re)和峰浓度比(Ce)。结果:左金丸组大鼠肝组织中盐酸小檗碱和盐酸巴马汀的药-时曲线下面积分别为(7 051.268±518.354),(2 179.139±195.617)ng·g-1·h,药峰浓度分别为(1 645.961±129.862),(383.889±19.526)ng·g-1,与黄连组比较显著增加。3种生物碱类成分的在肝中Re分别为1.338,1.475和1.065,Ce分别为1.548,1.751和1.369;肺中Re分别为0.985,0.780和0.930,Ce分别为0.834,1.051和0.905。结论:吴茱萸可能是通过增加黄连生物碱类成分在大鼠肝中分布和减少其在肺中分布来发挥引经作用。
OBJECTIVE: To investigate the effect of Evodia on the distribution kinetics of berberine hydrochloride, palmatine hydrochloride and jatrorrhizine hydrochloride in Zuojin Pills in rats, and to explore the mechanism of the administration of Evodia rutaecarpa. METHODS: Rats were given gavage of Coptis chinensis and Coptis chinensis Franch. The samples were collected at different time points after administration. The changes of the contents of three kinds of Coptis alkaloids in the tissues were determined by UPLC MS / MS. The DAS 2.0 software Tissue distribution kinetic parameters, relative uptake (Re) and peak concentration ratio (Ce) were calculated. Results: The area under the drug-time curve of berberine hydrochloride and palmatine hydrochloride in Zuojin pills group were (7 051.268 ± 518.354), (2 179.139 ± 195.617) ng · g -1 · h, respectively The peak concentrations were (1645.961 ± 129.862) and (383.889 ± 19.526) ng · g-1, respectively, which were significantly increased compared with Coptis. Re in the liver were 1.338, 1.475 and 1.065 for the three alkaloid components, 1.548, 1.751 and 1.369 for Ce, 0.985, 0.780 and 0.930 for Ce, 0.834, 1.051 and 0.905 for Ce, respectively. Conclusion: Fructus Evodiae may play an important role in promoting the distribution of Coptis alkaloids in rat liver and reducing its distribution in the lung.