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半薄切片(厚切片)有助于超薄切片的定位,以及光镜与电镜观察的密切结合,是应用电镜技术进行生物医学研究的手段之一。半薄切片制片过程中,在载玻片上展片与贴片及切片染色,是该技术的重要步骤。按传统的方法,展片时用酒精灯火焰加热,温度不易控制,且成批制片费时。贴片则是在玻片上涂蛋白甘油,长期保存易生霉,或置50～70℃烤箱中数小时,因空气导热慢,耗时较多,而染色通过火焰加热,易沸腾造成过染或沉淀。因此,我室用自动恒温电熨斗试制了一种半薄切片调温烤片装置(易制作、成
Semi-thin section (thick section) helps the positioning of ultra-thin sections and the close combination of light and electron microscope observation. It is one of the means to apply biomedical research by electron microscopy. Semi-thin slice production process, the slide on the slide and patch and slice staining, is an important step in this technology. According to the traditional method, when the film is heated with alcohol lamp flame, the temperature is not easy to control, and time-consuming batch production is required. Patch is coated on glass slides glycerol, long-term preservation of moldy, or set 50 ~ 70 ℃ oven for several hours, due to air conduction slow, more time-consuming, and dyeing through the flame heating, easy to cause excessive boiling or boiling precipitation. Therefore, my room with a trial of a thermostat electric iron a semi-thin slice thermostat device (easy to make into